Hydrogen peroxide biosensor based on the immobilization of horseradish peroxidase onto a poly(aniline-co-N-methylthionine) film

被引：44
作者：
Chen, Chuanxiang [1 ]
Hong, Xiaozhang [1 ]
Xu, Tingting [1 ]
Chen, Ankang [1 ]
Lu, Lin [1 ]
Gao, Yuhua [1 ]
机构：
[1] Jiangsu Univ Sci & Technol, Sch Environm & Chem Engn, 2 Mengxi Rd, Zhenjiang 212003, Peoples R China
来源：
SYNTHETIC METALS | 2016年 / 212卷
基金：
中国国家自然科学基金;
关键词：
Hydrogen peroxide; Hydrogen peroxidase; Biosensor; Poly(aniline-co-N-methylthionine); BOVINE SERUM-ALBUMIN; BIOELECTROCHEMICAL RESPONSE; AMPEROMETRIC BIOSENSOR; CHOLESTEROL BIOSENSOR; CONDUCTING POLYMERS; OXIDASE ELECTRODE; GLUCOSE-OXIDASE; POLYANILINE; COMPOSITE; ELECTROSYNTHESIS;
D O I：
10.1016/j.synthmet.2015.12.012
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
In this present work, we developed a novel hydrogen peroxide (H2O2) biosensor fabricated using electrochemical doping to immobilize hydrogen peroxidase (HRP) in a new conducting polymer, poly (aniline-co-N-methylthionine) (PAN-PNMThH). Amperometric detection of H2O2 was evaluated by holding the PAN-PNMThH HRP electrode at -0.25 V (versus saturated calomel electrode (SCE)). PAN-PNMThH showed excellent redox activity and high porosity and acted as an electron transfer mediator. The biosensor had a wide linear response range from 5.0 mu m to 60.0 mM H2O2 with a sensitivity of 35 mA M-1 cm(-2), a detection limit of 3.2 mu M (signal-to-noise ratio of 3) and an apparent Michaelis constant (K-M) of 2.79 mM. The biosensor possessed good analytical performance and storage stability. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：123 / 130
页数：8
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