Comparison of Different Protein Concentration Strategies by Two-Dimensional Gel Electrophoresis of Human Serum Proteomics

被引：0

作者：

Liu, Wentao ^{[1
]}

Yang, Ling ^{[1
]}

Liu, Bingya ^{[1
]}

Cai, Qu ^{[1
]}

Zhang, Yi ^{[1
]}

Chen, Xuehua ^{[1
]}

Yang, Qiumeng ^{[1
]}

Zhu, Zhenggang ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Ruijin Hosp, Dept Surg, Shanghai Inst Digest Surg, Shanghai 200025, Peoples R China

来源：

ASIAN JOURNAL OF CHEMISTRY | 2010年 / 22卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Serum; Biomarker; Two-dimensional gel electrophrosis; Freeze-drying; Ultrafiltration; SAMPLE PREPARATION;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the research of serum proteomics, an appropriate sample preparation is essential for obtaining good results in two dimensional electrophoresis (2-DE). The concentration steps are necessary to sample preparation and several other processes. Our goal is to find the most efficient method that minimally impacted on serum proteins for a serum proteomics. We compared three strategies of concentration including ReadyPrep 2-D cleanup kit, freeze-drying and ultrafiltration using crude serum samples and high abundant proteins depleted serum samples. By comparing the different characteristics of the operation process, the recovery effect and the compatibility with 2-DE, it is found that the freeze-drying and ultrafiltration performed an efficient sample concentration of serum proteins. And all of these three concentration strategies delivered a satisfied compatibility with 2-DE technique of serum proteomics. Less manual operations involved in concentration procedures was expected to provide a more reliable result of serum proteomics.

引用

页码：3107 / 3114

页数：8

共 14 条

[1] Clinical cancer proteomics: Promises and pitfalls [J].