Genomics tools for unraveling chromosome architecture

被引：156

作者：

van Steensel, Bas ^{[1
]}

Dekker, Job ^{[2
]}

机构：

[1] Netherlands Canc Inst, Div Gene Regulat, Amsterdam, Netherlands

[2] Univ Massachusetts, Sch Med, Program Gene Funct & Express, Dept Biochem & Mol Pharmacol, Worcester, MA USA

来源：

NATURE BIOTECHNOLOGY | 2010年 / 28卷 / 10期

基金：

美国国家卫生研究院;

关键词：

NUCLEAR LAMINA INTERACTIONS; BETA-GLOBIN LOCUS; CHROMATIN INTERACTIONS; CONFORMATION CAPTURE; GENE-EXPRESSION; DNA INTERACTIONS; PORE COMPLEXES; X-INACTIVATION; ORGANIZATION; TRANSCRIPTION;

D O I：

10.1038/nbt.1680

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The spatial organization of chromosomes inside the cell nucleus is still poorly understood. This organization is guided by intra- and interchromosomal contacts and by interactions of specific chromosomal loci with relatively fixed nuclear 'landmarks' such as the nuclear envelope and the nucleolus. Researchers have begun to use new molecular genome-wide mapping techniques to uncover both types of molecular interactions, providing insights into the fundamental principles of interphase chromosome folding.

引用

页码：1089 / 1095

页数：7

共 79 条

[1]

Bacher CP, 2006, NAT CELL BIOL, V8, P293, DOI 10.1038/ncb1365

[2] GENE GATING - A HYPOTHESIS [J].

BLOBEL, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (24) :8527-8529

[3] Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettes [J].

Bolzer, A ;

Kreth, G ;

Solovei, I ;

Koehler, D ;

Saracoglu, K ;

Fauth, C ;

Müller, S ;

Eils, R ;

Cremer, C ;

Speicher, MR ;

Cremer, T .

PLOS BIOLOGY, 2005, 3 (05) :826-842

[4] Global histone acetylation induces functional genomic reorganization at mammalian nuclear pore complexes [J].

Brown, Christopher R. ;

Kennedy, Caleb J. ;

Delmar, Valerie A. ;

Forbes, Douglass J. ;

Silver, Pamela A. .

GENES & DEVELOPMENT, 2008, 22 (05) :627-639

[5] Chromatin-Bound Nuclear Pore Components Regulate Gene Expression in Higher Eukaryotes [J].

Capelson, Maya ;

Liang, Yun ;

Schulte, Roberta ;

Mair, William ;

Wagner, Ulrich ;

Hetzer, Martin W. .

CELL, 2010, 140 (03) :372-U100

[6] Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization [J].

Casolari, JM ;

Brown, CR ;

Komili, S ;

West, J ;

Hieronymus, H ;

Silver, PA .

CELL, 2004, 117 (04) :427-439

[7] Moving chromatin within the interphase nucleus-controlled transitions? [J].

Chuang, Chien-Hui ;

Belmont, Andrew S. .

SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2007, 18 (05) :698-706

[8] The three 'C's of chromosome conformation capture: controls, controls, controls [J].

Dekker, J .

NATURE METHODS, 2006, 3 (01) :17-21

[9] Capturing chromosome conformation [J].

Dekker, J ;

Rippe, K ;

Dekker, M ;

Kleckner, N .

SCIENCE, 2002, 295 (5558) :1306-1311

[10] Mapping in Vivo Chromatin Interactions in Yeast Suggests an Extended Chromatin Fiber with Regional Variation in Compaction [J].

Dekker, Job .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (50) :34532-34540

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