Effect of dietary cholesterol and high fat on ceramide concentration in rat tissues

被引:29
作者
Ichi, Ikuyo [1 ]
Nakahara, Kayoko [1 ]
Kiso, Kaori [1 ]
Kojo, Shosuke [1 ]
机构
[1] Nara Womens Univ, Dept Food Sci & Nutr, Nara 630, Japan
关键词
atherosclerosis; ceramide; cholesterol; fat; electrospray tandem mass spectrometry; adipose; lipid;
D O I
10.1016/j.nut.2007.04.014
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Objective: Recent studies have indicated that plasma sphingomyelin levels and sphingomyelinase activity are risk factors for atherosclerosis. Therefore, it is suggested that ceramides, which are hydrolyzed products of sphingomyelin and a biologically active lipid causing apoptosis in a variety of cells, have an important role in the incidence of atherosclerosis. In this study, we examined whether cholesterol- and fat-enriched diets, which are causes of atherosclerosis, affect ceramide metabolism. In addition, we found a relation among lipid markers of atherosclerosis such as cholesterol, triacylglycerol, and ceramide concentrations. Methods: Male Wistar rats were fed a diet supplemented with 1% cholesterol or 30% high-fat diet for 8 wk. Tissue ceramide levels were analyzed using electrospray tandem mass spectrometry. Results: The major ceramides in plasma and the liver were C24:0 and C24:1. The major ceramides in adipose tissues were C16:0 and C24:0. Therefore, the ceramide composition of the adipose tissues was different from that of plasma and the liver. In addition, total ceramide levels in plasma and the adipose tissues of rats fed cholesterol were higher than those in the control group. Conclusion: The accumulation of cholesterol caused an increase in ceramides, which might be a new risk factor for atherosclerosis. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:570 / 574
页数:5
相关论文
共 37 条
  • [1] A COLORIMETRIC METHOD FOR ESTIMATING SERUM TRIGLYCERIDES
    FLETCHER, MJ
    [J]. CLINICA CHIMICA ACTA, 1968, 22 (03) : 393 - &
  • [2] FOLCH J, 1951, J BIOL CHEM, V191, P833
  • [3] FONG LG, 1987, J LIPID RES, V28, P1466
  • [4] Obesity in the new millennium
    Friedman, JM
    [J]. NATURE, 2000, 404 (6778) : 632 - 634
  • [5] GLEELEN MJH, 1995, J NUTR, V125, P2294
  • [6] GOLAY A, 1997, INT J OBES S3, V21, P2
  • [7] The ceramide-centric universe of lipid-mediated cell regulation: Stress encounters of the lipid kind.
    Hannun, YA
    Obeid, LM
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (29) : 25847 - 25850
  • [8] HEINECKE JW, 1993, J LIPID RES, V34, P2051
  • [9] Effect of myriocin on plasma sphingolipid metabolism and atherosclerosis in apoE-deficient mice
    Hojjati, MR
    Li, ZQ
    Zhou, HW
    Tang, SS
    Huan, CM
    Ooi, E
    Lu, SD
    Jiang, XC
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (11) : 10284 - 10289
  • [10] Physiology and pathophysiology of sphingolipid metabolism and signaling
    Huwiler, A
    Kolter, T
    Pfeilschifter, J
    Sandhoff, K
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2000, 1485 (2-3): : 63 - 99