Simple and sensitive detection of uracil-DNA glycosylase activity using dsDNA-templated copper nanoclusters as fluorescent probes

被引：17

作者：

Cao, Manman ^{[1
]}

Jin, Yan ^{[1
]}

Li, Baoxin ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710062, Peoples R China

来源：

ANALYTICAL METHODS | 2016年 / 8卷 / 22期

基金：

中国国家自然科学基金;

关键词：

BASE EXCISION-REPAIR; SWITCH-ON PROBE; ENZYME-ACTIVITY; G-QUADRUPLEX; SIGNAL AMPLIFICATION; ASSAY; NANOPARTICLES; LUMINESCENCE; APOPTOSIS; OXIDATION;

D O I：

10.1039/c6ay00900j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Sensitive detection of uracil-DNA glycosylase (UDG) activity is critical for the functional study of UDG and clinical diagnosis. Herein we exploit dsDNA-templated copper nanoclusters (CuNCs) as a fluorescent nano-dye for UDG assay. The UDG-catalyzed uracil removal can liberate ssDNA from the uracil-containing dsDNA substrate, and thus the introduction of Cu2+ and ascorbate cannot form the fluorescent CuNCs due to the lack of the dsDNA templates. By optimizing the number and location of uracil on the DNA strand, a low detection limit of 0.0005 UmL(-1) was achieved. The simple and sensitivemethodmay hold potential applications in UDG-related clinical diagnosis and functional research.

引用

收藏

页码：4319 / 4323

页数：5

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