Solid-Phase Synthesis of a Special Phosphorylated Peptide as a Biomarker for LC-MS/MS Detection for OPNA Exposure

被引：0

作者：

Li, Xinhai ^{[1
,2
]}

Yuan, Ling ^{[1
,2
]}

Wang, Qinggang ^{[3
]}

Liang, Longhui ^{[1
,2
]}

Huang, Guilan ^{[1
,2
]}

Liu, Shilei ^{[1
,2
]}

Liu, Jingquan ^{[1
]}

机构：

[1] State Key Lab NBC Protect Civilian, Beijing 102205, Peoples R China

[2] Res Inst Chem Def, Analyt Chem Lab, Beijing 102205, Peoples R China

[3] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, 189 Songling Rd, Qingdao 266101, Peoples R China

来源：

SYNLETT | 2017年 / 28卷 / 08期

关键词：

d(5)-VX; solid-phase; nonapeptide; isotope label; BuChE-OPNA adducts; TANDEM MASS-SPECTROMETRY; CHEMICAL WARFARE AGENTS; DEGRADATION-PRODUCTS; SARIN; SAMPLES; BUTYRYLCHOLINESTERASE; IDENTIFICATION; IONIZATION; ACIDS;

D O I：

10.1055/s-0036-1588691

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A synthesis of d(5)-VX adducted nonapeptide via solid-phase approach has been developed. The d(5)-VX peptide could be used as the isotope-labeled internal standard for LC-MS/MS detecting the BuChE-OPNA biomarkers. The Kaiser test was utilized to ensure the right connections of all of the amino acids. This method offers an access to the synthesis and detection of other phosphorylated nonapeptides.

引用

页码：986 / 988

页数：3

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