Coliphage as pressure surrogates for enteric viruses in foods

In this study the potential of using selected bacteriophages as pressure surrogates for hepatitis A virus (HAV) and Aichi virus (AiV) was investigated. The coliphages included, 14, MS2, Q beta, lambda imm 434, lambda cI 857 and K cI 857A. T-4 displayed similar pressure responses as HAV and was chosen for further study. The most pressure-resistant phage, MS2, was selected as a possible surrogate to estimate AiV inactivation by high pressure processing (HPP). HAV. AiV and their selected bacteriophage surrogates were treated at a range of pressures and times in three different media. All four were treated in phosphate-buffered saline (PBS), artificial seawater (ASW) or oyster slurry (OS) at 250, 400 or 500 MPa for 1, 5 or 10 min at 20 degrees C While T4 had similar pressure resistance to HAV under conditions of high (500 MPa) and lower pressure (250 MPa), inactivation trends were very different following treatment at 400 MPa and when the viruses were suspended in OS MS2 showed similar resistance as AN but at ambient treatment temperatures only The highest levels of inactivation of MS2 were achieved at 60 degrees C and 500 MPa. AiV was eliminated at 60 degrees C for 5 min at ambient pressure, but >3 log survived exposure to 60 degrees C at 500 MPa This degree of protection by pressure may be important in determining the mechanisms of pressure and heat resistances in other viruses. Industrial relevance Greater knowledge of the responses of viruses and their surrogates to high pressure will aid in the validation of new high pressure-processed food that may be at risk to contamination from HAV or other enteric viruses (C) 2009 Elsevier Ltd All rights reserved