Cloning and Identification of Salt Overly Sensitive (SOS1) Gene of Sugarcane

Salinity greatly affects gylcophyte plants such as sugarcane. It is one of the major factors that limit plant growth and consequently crop productivity. The aim of this study was to clone the full length of the SOS1 gene, a plasma membrane Na+/H+ antiporter and to characterize the gene through in silico characterization and real time quantitative PCR. Sugarcane, wild sugarcane, commercial cultivar (KPS94-13) and interspecific hybrid (biotec2) plants at the age of 1.5 months old were subjected to 1/10 Hoagland nutrient solution supplemented with 0, 100 and 200 mA/NaCI for 0-5 days. Total RNA from the leaves and roots of the plants were used as the template for first strand cDNA synthesis. The full length of SoSOS1 was 3,390 bp. The phylogenetic analysis revealed that putative SoSOS1 derived from KPS 94-13 was classified into the SOS1 group. The analysis of trans-membrane protein using TMPRED program showed 11 putative trans-membrane domains The analysis of SoSOS1 cDNA expression in the leaves and roots of the three sugarcane genotypes by real-time PCR revealed that the SoSOS1 cDNA expression levels in roots were higher than in leaves during 1-3 days period and subsequently the leaves were at higher levels than in roots during 4-5 days period at 100 mA4 NaCl stress. While at 200 mA4 NaCl stress the SoSOS1 cDNA expressions in leaves of the commercial cultivar and interspecific hybrid sugarcane were higher than in the roots. (C) 2018 Friends Science Publishers