Characterization of class I Newcastle disease virus isolates from Hong kong live bird markets and detection using real-time reverse transcription-PCR

被引：103
作者：
Kim, L. Mia
King, Daniel J.
Suarez, David L.
Wong, Chun W.
Afonso, Claudio L.
机构：
[1] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA
[2] Tai Lung Vet Lab, Agr Fisheries & Conservat Dept, Hong Kong, Hong Kong, Peoples R China
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2007年 / 45卷 / 04期
关键词：
D O I：
10.1128/JCM.02594-06
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.
引用
收藏
页码：1310 / 1314
页数：5
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