Characterization of class I Newcastle disease virus isolates from Hong kong live bird markets and detection using real-time reverse transcription-PCR

被引:103
作者
Kim, L. Mia
King, Daniel J.
Suarez, David L.
Wong, Chun W.
Afonso, Claudio L.
机构
[1] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA
[2] Tai Lung Vet Lab, Agr Fisheries & Conservat Dept, Hong Kong, Hong Kong, Peoples R China
关键词
D O I
10.1128/JCM.02594-06
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.
引用
收藏
页码:1310 / 1314
页数:5
相关论文
共 20 条
[1]   A molecular epidemiological study of avian paramyxovirus type 1 (Newcastle disease virus) isolates by phylogenetic analysis of a partial nucleotide sequence of the fusion protein gene [J].
Aldous, EW ;
Mynn, JK ;
Banks, J ;
Alexander, DJ .
AVIAN PATHOLOGY, 2003, 32 (03) :239-257
[2]   CHARACTERIZATION OF AN ANTIGENICALLY UNUSUAL VIRUS RESPONSIBLE FOR 2 OUTBREAKS OF NEWCASTLE-DISEASE IN THE REPUBLIC OF IRELAND IN 1990 [J].
ALEXANDER, DJ ;
CAMPBELL, G ;
MANVELL, RJ ;
COLLINS, MS ;
PARSONS, G ;
MCNULTY, MS .
VETERINARY RECORD, 1992, 130 (04) :65-68
[3]  
Alexander DJ, 2003, DIS POULTRY, P63
[4]   Identification and grouping of Newcastle disease virus strains by restriction site analysis of a region from the F gene [J].
BallagiPordany, A ;
Wehmann, E ;
Herczeg, J ;
Belak, S ;
Lomniczi, B .
ARCHIVES OF VIROLOGY, 1996, 141 (02) :243-261
[5]   Third genome size category of avian paramyxovirus serotype 1 (Newcastle disease virus) and evolutionary implications [J].
Czegledi, Aliz ;
Ujvari, Dorina ;
Somogyi, Eszter ;
Wehmann, Eniko ;
Werner, Ortrud ;
Lomniczi, Bela .
VIRUS RESEARCH, 2006, 120 (1-2) :36-48
[6]   Virulent Newcastle disease in Australia: Molecular epidemiological analysis of viruses isolated prior to and during the outbreaks of 1998-2000 [J].
Gould, AR ;
Kattenbelt, JA ;
Selleck, P ;
Hansson, E ;
Della-Porta, A ;
Westbury, HA .
VIRUS RESEARCH, 2001, 77 (01) :51-60
[7]  
HALL T. A., 1999, Nucleic Acids Symp Ser, V41, P95, DOI [10.1021/bk-1999-0734.ch008, DOI 10.1021/BK-1999-0734.CH008]
[8]   Two novel genetic groups (VIIb and VIII) responsible for recent Newcastle disease outbreaks in Southern Africa, one (VIIb) of which reached Southern Europe [J].
Herczeg, J ;
Wehmann, E ;
Bragg, RR ;
Dias, PMT ;
Hadjiev, G ;
Werner, O ;
Lomniczi, B .
ARCHIVES OF VIROLOGY, 1999, 144 (11) :2087-2099
[9]   Molecular characterization of Newcastle disease viruses isolated from recent outbreaks in Taiwan [J].
Ke, GM ;
Liu, HJ ;
Lin, MY ;
Chen, JH ;
Tsai, SS ;
Chang, PC .
JOURNAL OF VIROLOGICAL METHODS, 2001, 97 (1-2) :1-11
[10]   Effect of probe-site mismatches on detection of virulent Newcastle disease viruses using a fusion-gene real-time reverse transcription polymerase chain reaction test [J].
Kim, L. Mia ;
Afonso, Claudio L. ;
Suarez, David L. .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2006, 18 (06) :519-528