Fabrication of Protein Microarrays Using the Electrospray Deposition (ESD) Method: Application of Microfluidic Chips in Immunoassay

被引：6

作者：

Lee, Bumhwan ^{[1
,2
]}

Tajima, Akihiko ^{[1
]}

Kim, Joonwan ^{[2
]}

Yamagata, Yutaka ^{[3
]}

Nagamune, Teruyuki ^{[1
]}

机构：

[1] Sch Engn, Dept Chem & Biotechnol, Tokyo 1138656, Japan

[2] Tokyo Inst Technol, Precis & Intelligence Lab, Yokohama, Kanagawa 2268503, Japan

[3] RIKEN, VCAD Appl Fabricat Team, Saitama, Japan

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2010年 / 15卷 / 01期

关键词：

electrospray deposition method; protein microarray; biochip; high-throughput Immunoassay; GENE-EXPRESSION; ANTIBODIES; PATTERNS;

D O I：

10.1007/s12257-009-3033-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, antibody-based protein microarrays for high-throughput immunoassay were fabricated on an aldehyde-modified indium-tin oxide glass plate using the electrospray deposition (ESD) method and their characteristics were evaluated immunochemically. The microarrays were also integrated into microfluidic chips with a polydimethylsiloxane (PDMS) micro-channel to detect human cytokines, which were quantitatively analyzed with a high resolution charge-coupled device. Simultaneous detection of various antigens was performed using the microarrays with considerable sensitivity (ca. 100 pg/mL). The results of this study indicate that microfluidic chip comprising a protein microarray formed by the ESD method and a PDMS micro-channel could be easy to handle, and offers high-throughput detection of molecular biomarkers. (C) KSBB

引用

页码：145 / 151

页数：7

共 22 条

[1] Printing patterns of proteins
Bernard, A
Delamarche, E
Schmid, H
Michel, B
Bosshard, HR
Biebuyck, H
[J]. LANGMUIR, 1998, 14 (09) : 2225 - 2229
[2] BOGUSLAVSKY J, 2001, GENOMICS PROTEOMICS, V1, P46
[3] Antibodies in diagnostics - from immunoassays to protein chips
Borrebaeck, CAK
[J]. IMMUNOLOGY TODAY, 2000, 21 (08): : 379 - 382
[4] CYTOKINES IN T-CELL DEVELOPMENT
CARDING, SR
HAYDAY, AC
BOTTOMLY, K
[J]. IMMUNOLOGY TODAY, 1991, 12 (07): : 239 - 245
[5] Accessing genetic information with high-density DNA arrays
Chee, M
Yang, R
Hubbell, E
Berno, A
Huang, XC
Stern, D
Winkler, J
Lockhart, DJ
Morris, MS
Fodor, SPA
[J]. SCIENCE, 1996, 274 (5287) : 610 - 614
[6] Morphology control of thin LiCoO2 films fabricated using the electrostatic spray deposition (ESD) technique
Chen, CH
Kelder, EM
vanderPut, PJJM
Schoonman, J
[J]. JOURNAL OF MATERIALS CHEMISTRY, 1996, 6 (05) : 765 - 771
[7] Hatton RA, 2001, THIN SOLID FILMS, V394, P292, DOI 10.1016/S0040-6090(01)01191-9
[8] Electrostatic spraying: A novel technique for preparation of polymer coatings on electrodes
Hoyer, B
Sorensen, G
Jensen, N
Nielsen, DB
Larsen, B
[J]. ANALYTICAL CHEMISTRY, 1996, 68 (21) : 3840 - 3844
[9] IKE F, 2007, DETECTION EMERGING Z
[10] Direct and dry micro-patterning of nano-particles by electrospray deposition through a micro-stencil mask
Kim, J-W
Yamagata, Y.
Kim, B. J.
Higuchi, T.
[J]. JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2009, 19 (02)

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