Mass spectrometric detection of nodularin and desmethylnodularin in mussels and flounders

被引：48
作者：
Karlsson, K
Sipiä, V
Kankaanpää, H
Meriluoto, J
机构：
[1] Abo Akad Univ, Dept Biochem & Pharm, Turku 20521, Finland
[2] Finnish Inst Marine Res, Helsinki 00931, Finland
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 784卷 / 02期
基金：
芬兰科学院;
关键词：
mussel; flounder; nodularin; desmethylnodularin;
D O I：
10.1016/S1570-0232(02)00802-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Samples of mussels and flounders from the Baltic Sea were analysed for nodularin content on two different LC-MS instruments (triple quadrupole and ion trap). The triple quadrupole instrument was well suited for the quantitative analysis. The limit of detection in the selected ion recording mode was 5 pg and in the multiple reactant monitoring mode 500 pg on column for extracts of Nodularia spumigena. The fragmentation patterns of nodularin-R. and desmethylnodularin-R were recorded and shown to be similar to those of microcystins. LC-MS proved to be an excellent tool for the analyses of cyanobacterial hepatotoxins in complex matrices. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：243 / 253
页数：11
相关论文
共 35 条
  • [1] A molecular basis for different interactions of marine toxins with protein phosphatase-1 - Molecular models for bound motuporin, microcystins, okadaic acid, and calyculin A
    Bagu, JR
    Sykes, BD
    Craig, MM
    Holmes, CFB
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (08) : 5087 - 5097
  • [2] Molecular mechanisms underlying the interaction of motuporin and microycystins with type-1 and type-2A protein phosphatases
    Craig, M
    Luu, HA
    McCready, TL
    Williams, D
    Andersen, RJ
    Holmes, CFB
    [J]. BIOCHEMISTRY AND CELL BIOLOGY, 1996, 74 (04) : 569 - 578
  • [3] de Magalhaes VF, 2001, TOXICON, V39, P1077
  • [4] ANALYSIS OF MICROCYSTINS FROM CYANOBACTERIA BY LIQUID-CHROMATOGRAPHY WITH MASS-SPECTROMETRY USING ATMOSPHERIC-PRESSURE IONIZATION
    EDWARDS, C
    LAWTON, LA
    BEATTIE, KA
    CODD, GA
    PLEASANCE, S
    DEAR, GJ
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993, 7 (08) : 714 - 721
  • [5] Sensitive detection of apoptogenic toxins in suspension cultures of rat and salmon hepatocytes
    Fladmark, KE
    Serres, MH
    Larsen, NL
    Yasumoto, T
    Aune, T
    Doskeland, SO
    [J]. TOXICON, 1998, 36 (08) : 1101 - 1114
  • [6] Francis G., 1878, Nature, V18, P11, DOI DOI 10.1038/018011D0
  • [7] Simultaneous determination of the cyanotoxins anatoxin A, microcystin desmethyl-3, LR, RR, and YR in fish muscle using liquid chromatography-mass spectrometry
    Hormazábal, V
    Ostensvik, O
    Underdal, B
    Skulberg, OM
    [J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2000, 23 (02) : 185 - 196
  • [8] Chromatographic and spectral behaviour and detection of hepatotoxic nodularin in fish, clam, mussel and mouse tissues using HPLC analysis
    Kankaanpää, HT
    Vuorensola, KM
    Sipiä, VO
    Meriluoto, JAO
    [J]. CHROMATOGRAPHIA, 2002, 55 (3-4) : 157 - 162
  • [9] Detection and identification of metabolites of microcystins formed in vivo in mouse and rat livers
    Kondo, F
    Matsumoto, H
    Yamada, S
    Ishikawa, N
    Ito, E
    Nagata, S
    Ueno, Y
    Suzuki, M
    Harada, K
    [J]. CHEMICAL RESEARCH IN TOXICOLOGY, 1996, 9 (08) : 1355 - 1359
  • [10] FORMATION, CHARACTERIZATION, AND TOXICITY OF THE GLUTATHIONE AND CYSTEINE CONJUGATES OF TOXIC HEPTAPEPTIDE MICROCYSTINS
    KONDO, F
    IKAI, Y
    OKA, H
    OKUMURA, M
    ISHIKAWA, N
    HARADA, K
    MATSUURA, K
    MURATA, H
    SUZUKI, M
    [J]. CHEMICAL RESEARCH IN TOXICOLOGY, 1992, 5 (05) : 591 - 596
1234