Molecular Confirmation of the Relationship between Candida guilliermondii Fks1p Naturally Occurring Amino Acid Substitutions and Its Intrinsic Reduced Echinocandin Susceptibility

被引：0

作者：

Dudiuk, Catiana ^{[1
,2
]}

Macedo, Daiana ^{[1
]}

Leonardelli, Florencia ^{[1
,2
]}

Theill, Laura ^{[1
]}

Cabeza, Matias S. ^{[1
,2
]}

Gamarra, Soledad ^{[1
]}

Garcia-Effron, Guillermo ^{[1
,2
]}

机构：

[1] Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, Catedra Parasitol & Micol, Lab Micol & Diagnost Mol, Santa Fe, Argentina

[2] Ctr Cient Tecnol Santa Fe, Consejo Nacl Invest Cient & Tecnol, Santa Fe, Argentina

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2017年 / 61卷 / 05期

关键词：

Candida guilliermondii; FKS mutations; echinocandin resistance; molecular mechanism; INVASIVE CANDIDIASIS; SACCHAROMYCES-CEREVISIAE; ANIDULAFUNGIN; SURVEILLANCE; FLUCONAZOLE; RESISTANCE; THERAPY; PARAPSILOSIS; CASPOFUNGIN; MICAFUNGIN;

D O I：

10.1128/AAC.02644-16

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Candida guilliermondii shows intrinsic reduced echinocandin susceptibility. It harbors two polymorphisms (L633M and T634A) in the Fks1p hot spot 1 region. Our objective was to confirm that the reduced echinocandin susceptibility of C. guilliermondii is due to those naturally occurring substitutions. We constructed a Saccharomyces cerevisiae mutant in which a region of the FKS1 gene (including hot spot 1) was replaced with that from C. guilliermondii. The chimeric mutants showed 32-fold increases in echinocandin MIC values, confirming the hypothesis.

引用

页数：5

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