Molecular Confirmation of the Relationship between Candida guilliermondii Fks1p Naturally Occurring Amino Acid Substitutions and Its Intrinsic Reduced Echinocandin Susceptibility

被引：0
作者：
Dudiuk, Catiana [1 ,2 ]
Macedo, Daiana [1 ]
Leonardelli, Florencia [1 ,2 ]
Theill, Laura [1 ]
Cabeza, Matias S. [1 ,2 ]
Gamarra, Soledad [1 ]
Garcia-Effron, Guillermo [1 ,2 ]
机构：
[1] Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, Catedra Parasitol & Micol, Lab Micol & Diagnost Mol, Santa Fe, Argentina
[2] Ctr Cient Tecnol Santa Fe, Consejo Nacl Invest Cient & Tecnol, Santa Fe, Argentina
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2017年 / 61卷 / 05期
关键词：
Candida guilliermondii; FKS mutations; echinocandin resistance; molecular mechanism; INVASIVE CANDIDIASIS; SACCHAROMYCES-CEREVISIAE; ANIDULAFUNGIN; SURVEILLANCE; FLUCONAZOLE; RESISTANCE; THERAPY; PARAPSILOSIS; CASPOFUNGIN; MICAFUNGIN;
D O I：
10.1128/AAC.02644-16
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Candida guilliermondii shows intrinsic reduced echinocandin susceptibility. It harbors two polymorphisms (L633M and T634A) in the Fks1p hot spot 1 region. Our objective was to confirm that the reduced echinocandin susceptibility of C. guilliermondii is due to those naturally occurring substitutions. We constructed a Saccharomyces cerevisiae mutant in which a region of the FKS1 gene (including hot spot 1) was replaced with that from C. guilliermondii. The chimeric mutants showed 32-fold increases in echinocandin MIC values, confirming the hypothesis.
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页数：5
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