Dexamethasone reverses TGF-β-mediated inhibition of primary rat preadipocyte differentiation

被引：19

作者：

Shin, SM ^{[1
]}

Kim, KY ^{[1
]}

Kim, JK ^{[1
]}

Yoon, SR ^{[1
]}

Choi, I ^{[1
]}

Yang, Y ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Immunol Lab, Taejon 305333, South Korea

来源：

FEBS LETTERS | 2003年 / 543卷 / 1-3期

关键词：

dexamethasone; rat preadipocyte; transforming growth factor-beta; CCAAT/enhancer binding protein-alpha; peroxisome proliferator-activated receptor gamma;

D O I：

10.1016/S0014-5793(03)00371-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dexamethasone and transforming growth factor-beta (TGF-beta) show contrary effects on differentiation of adipocytes. Dexamethasone stimulates adipocyte differentiation whereas TGF-beta inhibits it. In the present study, we investigated whether dexamethasone could reverse the TGF-beta-mediated inhibition of preadipocyte differentiation. Primary rat preadipocytes, obtained from Sprague-Dawley rats, were pretreated with dexamethasone in the presence or absence of TGF-beta, prior to the induction of differentiation. Co-treatment of dexamethasone and TGF-beta before inducing differentiation reversed the TGF-beta-mediated inhibition of preadipocyte differentiation. In order to elucidate the mechanism by which dexamethasone reversed the effect of TGF-beta on the inhibition of preadipocyte differentiation, the expression of CCAAT/enhancer binding protein-alpha (C/EBPalpha) and peroxisome proliferator-activated receptorgamma(PPARgamma) was examined. Dexamethasone increased C/EBPalpha and PPARgamma expression in the absence of TGF-beta and also recovered the TGF-beta-mediated suppression of C/EBPalpha expression in preadipocytes. Its effect was sustained in differentiated adipocytes as well. However, those effects were not observed in 3T3-L1 preadipocytes or differentiated adipocytes. These results indicate that dexamethasone reverses the TGF-beta-mediated suppression of adipocyte differentiation by regulating the expression of C/EBPalpha and PPARgamma, which is dependent on the cellular context. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：25 / 30

页数：6

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