Molecular cloning and characterization of a mitogen-activated protein kinase kinase (OoMAPKK1) in Origanum onites L. (Lamiaceae)

被引:2
作者
Poyraz, Ismail [1 ]
机构
[1] Bilecik Seyh Edebali Univ, Fac Letters & Sci, Mol Biol & Genet Dept, TR-11210 Bilecik, Turkey
关键词
MAPKK; Gene cloning; Origanum onites L; Abiotic stresses; Semi-quantitative RT-PCR; MEDIATES OSMOTIC-STRESS; MAP KINASE; ESSENTIAL OILS; PLANT MAPK; GENE; PATHWAYS;
D O I
10.1007/s13562-013-0238-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plants are constantly exposed to a variety of environmental (biotic and abiotic) stresses and they develop sophisticated signaling pathways to adapt the changing environment. Mitogen activated protein kinase (MAPK) cascade in plants is a common mechanism used to translate the external stimuli into cellular responses and composed of triple kinases modules namely MAPKKK-MAPKK-MAPK. In this study, a MAPKK (OoMAPKK1) has been cloned from Origanum onites L. (Lamiaceae) which is a commercially cultivated aromatic plant in the world. The OoMAPKK1 gene is 1,059 bp long, encodes a 353 amino acid protein with an estimated molecular mass of 39.416 kDa. The secondary structure of the OoMAPKK1 protein was analyzed by SOPMA and the 3D crystal structure of OoMAPKK1 protein was created by using Cn3D 4.1 software. The expression levels of OoMAPKK1 under different abiotic stresses (cold, wounding and abscisic acid) were investigated by semi-quantitative RT-PCR. Changes in the expression of OoMAPKK1 under cold stress, wounding and abscisic acid (ABA) application may suggest that OoMAPKK1 is involved in the cross-talk of multi-environmental stress responses.
引用
收藏
页码:75 / 83
页数:9
相关论文
共 36 条
[1]   Composition and antimicrobial activity of the essential oils of two Origanum species [J].
Aligiannis, N ;
Kalpoutzakis, E ;
Mitaku, S ;
Chinou, IB .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (09) :4168-4170
[2]  
Baser K. H. C., 1993, Journal of Essential Oil Research, V5, P619
[3]  
Baydar H., 2005, Akdeniz Univ. J. Fac. Agricult, V18, P175
[4]   Stressing the role of MAP kinases in mitogenic stimulation [J].
Bögre, L ;
Meskiene, I ;
Heberle-Bors, E ;
Hirt, H .
PLANT MOLECULAR BIOLOGY, 2000, 43 (5-6) :705-718
[5]   Environmental factors affecting chemical variability of essential oils in Thymus piperella L. [J].
Boira, H ;
Blanquer, A .
BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 1998, 26 (08) :811-822
[6]   Role of nicotianamine in the intracellular delivery of metals and plant reproductive development [J].
Takahashi, M ;
Terada, Y ;
Nakai, I ;
Nakanishi, H ;
Yoshimura, E ;
Mori, S ;
Nishizawa, NK .
PLANT CELL, 2003, 15 (06) :1263-1280
[7]   Exploring the evolutionary path of plant MAPK networks [J].
Doczi, Robert ;
Okresz, Laszlo ;
Romero, Alfonso E. ;
Paccanaro, Alberto ;
Boegre, Laszlo .
TRENDS IN PLANT SCIENCE, 2012, 17 (09) :518-525
[8]   Gene regulation by MAP kinase cascades [J].
Fiil, Berthe Katrine ;
Petersen, Klaus ;
Petersen, Morten ;
Mundy, John .
CURRENT OPINION IN PLANT BIOLOGY, 2009, 12 (05) :615-621
[9]   Ancient signals: comparative genomics of plant MAPK and MAPKK gene families [J].
Hamel, Louis-Philippe ;
Nicole, Marie-Claude ;
Sritubtim, Somrudee ;
Morency, Marie-Jose ;
Ellis, Margaret ;
Ehlting, Juergen ;
Beaudoin, Nathalie ;
Barbazuk, Brad ;
Klessig, Dan ;
Lee, Justin ;
Martin, Greg ;
Mundy, John ;
Ohashi, Yuko ;
Scheel, Dierk ;
Sheen, Jen ;
Xing, Tim ;
Zhang, Shuqun ;
Seguin, Armand ;
Ellis, Brian E. .
TRENDS IN PLANT SCIENCE, 2006, 11 (04) :192-198
[10]  
Ichimura K, 1998, DNA Res, V5, P341, DOI 10.1093/dnares/5.6.341