Analysis of chlorinated phenols in animal organs

Chronic latent shortage of natural antioxidants in the diet of experimental animals has been shown to enhance toxic effect of dichlorophenol. For the experimental studies, a GC method for the determination of 2,4-dichlorophenol in organs and blood serum of guinea pigs has been developed. The method is based on hydrolytic splitting of the conjugates prior to isolation of the compound from the organs by distillation with dichloromethane. For isolation from the serum, solid-phase extraction on Empore extraction discs was employed. The recoveries for a,4-dichlorophenol contents of 0.005 to 10.0 mug g(-1) in the tissues were between 81.2 % and 97.3 %, and between 89.9 % and 95.1 % for the content range of 0.01 to 1.0 mug g(-1) in serum. The LOQ of the method is 5 x 10(-4) mug g(-1), the relative standard deviation was below 7 %. The results of the study indicated lower accumulation rate of dichlorophenols in animals fed by higher doses of ascorbic acid.