New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response

被引:9
作者
Adda-Rezig, Hanane [1 ]
Carron, Clemence [1 ]
de Barros, Jean-Paul Pais [2 ]
Choubley, Helene [2 ]
Charron, Emilie [2 ]
Rerole, Anne-Laure [2 ]
Laheurte, Caroline [1 ,3 ]
Louvat, Pascale [1 ,3 ]
Gaiffe, Emilie [1 ,4 ]
Simula-Faivre, Dominique [5 ]
Deckert, Valerie [2 ]
Lagrost, Laurent [2 ]
Saas, Philippe [1 ,3 ,4 ]
Ducloux, Didier [1 ,4 ,5 ]
Bamoulid, Jamal [1 ,5 ]
机构
[1] Univ Bourgogne Franche Comte, Federat Hosp Univ INCREASE, RIGHT Interact Hote Greffon Tumeur Ingn Cellulair, UMR1098,LabEx LipST,INSERM,EFS BFC, Besancon, France
[2] Univ Bourgogne Franche Comte, LNC UMR1231, INSERM, LabEx LipSTIC, Dijon, France
[3] EFS Bourgogne Franche Comte, Plateforme BioMonitoring, Besancon, France
[4] Univ Hosp Besancon, Federat Hosp Univ INCREASE, Clin Invest Ctr Biotherapy, INSERM CIC1431,LabEx LipSTIC, Besancon, France
[5] Univ Hosp Besancon, Dept Nephrol Dialysis & Renal Transplantat, Besancon, France
关键词
end-stage renal disease; gut bacterial translocation; lipid A (LPS); HDL; cholesterol; chronic inflammation; ACID-BINDING PROTEIN; TLR4; ZONULIN; INNATE; CKD; TRANSPLANTATION; IDENTIFICATION; RECOGNITION; MICROBIOME; ACTIVATION;
D O I
10.3389/fimmu.2021.658404
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation. Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length. This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV). We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length. Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity. Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis. To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures. Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS. TLR4 or LPS antagonists decreased LPS-induced cytokine production by monocytes, demonstrating an LPS-specific effect. Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
引用
收藏
页数:14
相关论文
共 56 条
[1]   The Uremic Toxin Indoxyl Sulphate Enhances Macrophage Response to LPS [J].
Adesso, Simona ;
Popolo, Ada ;
Bianco, Giuseppe ;
Sorrentino, Rosalinda ;
Pinto, Aldo ;
Autore, Giuseppina ;
Marzocco, Stefania .
PLOS ONE, 2013, 8 (09)
[2]   Serum I-FABP as marker for enterocyte damage in coeliac disease and its relation to villous atrophy and circulating autoantibodies [J].
Adriaanse, M. P. M. ;
Tack, G. J. ;
Passos, V. Lima ;
Damoiseaux, J. G. M. C. ;
Schreurs, M. W. J. ;
van Wijck, K. ;
Riedl, R. G. ;
Masclee, A. A. M. ;
Buurman, W. A. ;
Mulder, C. J. J. ;
Vreugdenhil, A. C. E. .
ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2013, 37 (04) :482-490
[3]   Intestinal Inflammation Targets Cancer-Inducing Activity of the Microbiota [J].
Arthur, Janelle C. ;
Perez-Chanona, Ernesto ;
Muehlbauer, Marcus ;
Tomkovich, Sarah ;
Uronis, Joshua M. ;
Fan, Ting-Jia ;
Campbell, Barry J. ;
Abujamel, Turki ;
Dogan, Belgin ;
Rogers, Arlin B. ;
Rhodes, Jonathan M. ;
Stintzi, Alain ;
Simpson, Kenneth W. ;
Hansen, Jonathan J. ;
Keku, Temitope O. ;
Fodor, Anthony A. ;
Jobin, Christian .
SCIENCE, 2012, 338 (6103) :120-123
[4]   Intestinal barrier function [J].
Baumgart, DC ;
Dignass, AU .
CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE, 2002, 5 (06) :685-694
[5]  
Bertani Blake, 2018, EcoSal Plus, V8, DOI 10.1128/ecosalplus.ESP-0001-2018
[6]   SR-BI protects against endotoxemia in mice through its roles in glucocorticoid production and hepatic clearance [J].
Cai, Lei ;
Ji, Ailing ;
de Beer, Frederick C. ;
Tannock, Lisa R. ;
van der Westhuyzen, Deneys R. .
JOURNAL OF CLINICAL INVESTIGATION, 2008, 118 (01) :364-375
[7]   End-Stage Renal Disease-Associated Gut Bacterial Translocation: Evolution and Impact on Chronic Inflammation and Acute Rejection After Renal Transplantation [J].
Carron, Clemence ;
de Barros, Jean-Paul Pais ;
Gaiffe, Emilie ;
Deckert, Valerie ;
Adda-Rezig, Hanane ;
Roubiou, Caroline ;
Laheurte, Caroline ;
Masson, David ;
Simula-Faivre, Dominique ;
Louvat, Pascale ;
Moulin, Bruno ;
Frimat, Luc ;
Rieu, Philippe ;
Mousson, Christiane ;
Durrbach, Antoine ;
Heng, Anne-Elisabeth ;
Saas, Philippe ;
Ducloux, Didier ;
Lagrost, Laurent ;
Bamoulid, Jamal .
FRONTIERS IN IMMUNOLOGY, 2019, 10
[8]   HDL in innate and adaptive immunity [J].
Catapano, Alberico Luigi ;
Pirillo, Angela ;
Bonacina, Fabrizia ;
Norata, Giuseppe Danilo .
CARDIOVASCULAR RESEARCH, 2014, 103 (03) :372-383
[9]   The Role of Carbohydrates in the Lipopolysaccharide (LPS)/Toll-Like Receptor 4 (TLR4) Signalling [J].
Cochet, Florent ;
Peri, Francesco .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2017, 18 (11)
[10]   Improved quantification of plasma lipopolysaccharide (LPS) burden in sepsis using 3-hydroxy myristate (3HM): a cohort study [J].
Dargent, Auguste ;
De Barros, Jean-Paul Pais ;
Ksiazek, Elea ;
Fournel, Isabelle ;
Dusuel, Alois ;
Rerole, Anne Laure ;
Choubley, Helene ;
Masson, David ;
Lagrost, Laurent ;
Quenot, Jean-Pierre .
INTENSIVE CARE MEDICINE, 2019, 45 (11) :1678-1680