Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device

被引：57

作者：

Chueh, Bor-han

Zheng, Ying

Torisawa, Yu-suke

Hsiao, Amy Y.

Ge, Chunxi

Hsiong, Susan ^{[2
]}

Huebsch, Nathaniel ^{[2
,3
]}

Franceschi, Renny

Mooney, David J. ^{[2
]}

Takayama, Shuichi ^{[1
]}

机构：

[1] Univ Michigan, Macromol Sci & Engn Program, Ann Arbor, MI 48109 USA

[2] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02115 USA

[3] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA

来源：

BIOMEDICAL MICRODEVICES | 2010年 / 12卷 / 01期

关键词：

Microfluidics; Alginate; Hydrogel; Endothelial cells; Osteoblastic cells; Caged calcium; STOP-FLOW LITHOGRAPHY; MICROSTRUCTURES; MORPHOGENESIS; FABRICATION; PROTEINS;

D O I：

10.1007/s10544-009-9369-6

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This paper describes a simple reversible hydrogel patterning method for 3D cell culture. Alginate gel is formed in select regions of a microfluidic device through light-triggered release of caged calcium. In the pre-gelled alginate solution, calcium is chelated by DM-nitrophen (DM-n) to prevent cross-linking of alginate. After sufficient UV exposure the caged calcium is released from DM-n causing alginate to cross-link. The effect of using different concentrations of calcium and chelating agents as well as the duration of UV exposure is described. Since the cross-linking is based on calcium concentration, the cross-linked alginate can easily be dissolved by EDTA. We also demonstrate application of this capability to patterned microscale 3D co-culture using endothelial cells and osteoblastic cells in a microchannel.

引用

页码：145 / 151

页数：7

共 43 条

[1] Cell-interactive alginate hydrogels for bone tissue engineering [J].