A Comparative Proteomic Analysis Reveals a New Bi-Lobe Protein Required for Bi-Lobe Duplication and Cell Division in Trypanosoma brucei

被引：51

作者：

Zhou, Qing ^{[1
]}

Gheiratmand, Ladan ^{[1
]}

Chen, Yixin ^{[2
]}

Lim, Teck Kwang ^{[1
]}

Zhang, Jun ^{[2
]}

Li, Shaowei ^{[2
]}

Xia, Ningshao ^{[2
]}

Liu, Binghai ^{[1
]}

Lin, Qingsong ^{[1
]}

He, Cynthia Y. ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore

[2] Xiamen Univ, Sch Life Sci, Xiamen, Peoples R China

来源：

PLOS ONE | 2010年 / 5卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

FLAGELLUM ATTACHMENT; AFRICAN TRYPANOSOMES; GOLGI DUPLICATION; CYTOKINESIS; SEGREGATION; MEMBRANE; CYTOSKELETAL; EXPRESSION; DYNAMICS; CENTRIN;

D O I：

10.1371/journal.pone.0009660

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A Golgi-associated bi-lobed structure was previously found to be important for Golgi duplication and cell division in Trypanosoma brucei. To further understand its functions, comparative proteomics was performed on extracted flagellar complexes (including the flagellum and flagellum-associated structures such as the basal bodies and the bi-lobe) and purified flagella to identify new bi-lobe proteins. A leucine-rich repeats containing protein, TbLRRP1, was characterized as a new bi-lobe component. The anterior part of the TbLRRP1-labeled bi-lobe is adjacent to the single Golgi apparatus, and the posterior side is tightly associated with the flagellar pocket collar marked by TbBILBO1. Inducible depletion of TbLRRP1 by RNA interference inhibited duplication of the bi-lobe as well as the adjacent Golgi apparatus and flagellar pocket collar. Formation of a new flagellum attachment zone and subsequent cell division were also inhibited, suggesting a central role of bi-lobe in Golgi, flagellar pocket collar and flagellum attachment zone biogenesis.

引用

页数：12