Spoligotyping profile change caused by deletion of a direct variable repeat in a Mycobacterium tuberculosis isogenic laboratory strain

被引:14
作者
Aranaz, A [1 ]
Romero, B [1 ]
Montero, N [1 ]
Alvarez, J [1 ]
Bezos, J [1 ]
de Juan, L [1 ]
Mateos, A [1 ]
Domínguez, L [1 ]
机构
[1] UCM, Fac Vet, Dept Sanidad Anim, Madrid 28040, Spain
关键词
D O I
10.1128/JCM.42.11.5388-5391.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Spoligotyping is a major tool for molecular typing of Mycobacterium tuberculosis complex organisms. For epidemiological purposes, strains are considered clonal only when their spoligotyping patterns are identical. We report a change in the spoligotyping profiles of truly isogenic strains (a clinical isolate and a subculture derived in the laboratory) caused by deletion of a direct variable repeat. Without the information about the relationship between them, a link between these strains would have gone unnoticed. Evolutionary events should be taken into account in the interpretation of spoligotyping results and in the design of databases.
引用
收藏
页码:5388 / 5391
页数:4
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