Spoligotyping profile change caused by deletion of a direct variable repeat in a Mycobacterium tuberculosis isogenic laboratory strain

被引:14
作者
Aranaz, A [1 ]
Romero, B [1 ]
Montero, N [1 ]
Alvarez, J [1 ]
Bezos, J [1 ]
de Juan, L [1 ]
Mateos, A [1 ]
Domínguez, L [1 ]
机构
[1] UCM, Fac Vet, Dept Sanidad Anim, Madrid 28040, Spain
关键词
D O I
10.1128/JCM.42.11.5388-5391.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Spoligotyping is a major tool for molecular typing of Mycobacterium tuberculosis complex organisms. For epidemiological purposes, strains are considered clonal only when their spoligotyping patterns are identical. We report a change in the spoligotyping profiles of truly isogenic strains (a clinical isolate and a subculture derived in the laboratory) caused by deletion of a direct variable repeat. Without the information about the relationship between them, a link between these strains would have gone unnoticed. Evolutionary events should be taken into account in the interpretation of spoligotyping results and in the design of databases.
引用
收藏
页码:5388 / 5391
页数:4
相关论文
共 35 条
  • [1] Genotypic analysis of Mycobacterium tuberculosis in Bangladesh and prevalence of the Beijing strain
    Banu, S
    Gordon, SV
    Palmer, S
    Islam, R
    Ahmed, S
    Alam, KM
    Cole, ST
    Brosch, R
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (02) : 674 - 682
  • [2] Usefulness of spoligotyping to discriminate IS6110 low-copy-number Mycobacterium tuberculosis complex strains cultured in Denmark
    Bauer, J
    Andersen, ÅB
    Kremer, K
    Miörner, H
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (08) : 2602 - 2606
  • [3] Identification of a W variant outbreak of Mycobacterium tuberculosis via population-based molecular epidemiology
    Bifani, PJ
    Mathema, B
    Liu, ZY
    Moghazeh, SL
    Shopsin, B
    Tempalski, B
    Driscoll, J
    Frothingham, R
    Musser, JM
    Alcabes, P
    Kreiswirth, BN
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1999, 282 (24): : 2321 - 2327
  • [4] Epidemiological evidence of the spread of a Mycobacterium tuberculosis strain of the Beijing genotype on Gran Canaria Island
    Caminero, JA
    Pena, MJ
    Campos-Herrero, MI
    Rodríguez, JC
    Garcia, I
    Cabrera, P
    Lafoz, C
    Samper, S
    Takiff, H
    Afonso, O
    Pavón, JM
    Torres, MJ
    van Soolingen, D
    Enarson, DA
    Martin, C
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2001, 164 (07) : 1165 - 1170
  • [5] AN EVALUATION OF 1-HEXADECYLPYRIDINIUM CHLORIDE AS A DECONTAMINANT IN THE PRIMARY ISOLATION OF MYCOBACTERIUM-BOVIS FROM BOVINE LESIONS
    CORNER, LA
    TRAJSTMAN, AC
    [J]. VETERINARY MICROBIOLOGY, 1988, 18 (02) : 127 - 134
  • [6] delaSalmoniere YOG, 1997, J CLIN MICROBIOL, V35, P2210
  • [7] DNA typing of a nonviable culture of Mycobacterium tuberculosis in a homeless shelter outbreak
    Driscoll, JR
    McGarry, MA
    Taber, HW
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (01) : 274 - 275
  • [8] IS6110 transposition and evolutionary scenario of the direct repeat locus in a group of closely related Mycobacterium tuberculosis strains
    Fang, Z
    Morrison, N
    Watt, B
    Doig, C
    Forbes, KJ
    [J]. JOURNAL OF BACTERIOLOGY, 1998, 180 (08) : 2102 - 2109
  • [9] Snapshot of moving and expanding clones of Mycobacterium tuberculosis and their global distribution assessed by spoligotyping in an international study
    Filliol, I
    Driscoll, JR
    van Soolingen, D
    Kreiswirth, BN
    Kremer, K
    Valétudie, G
    Anh, DD
    Barlow, R
    Banerjee, D
    Bifani, PJ
    Brudey, K
    Cataldi, A
    Cooksey, RC
    Cousins, DV
    Dale, JW
    Dellagostin, OA
    Drobniewski, F
    Engelmann, G
    Ferdinand, S
    Gascoyne-Binzi, D
    Gordon, M
    Gutierrez, MC
    Haas, WH
    Heersma, H
    Kassa-Kelembho, E
    Ly, HM
    Makristathis, A
    Mammina, C
    Martin, G
    Moström, P
    Mokrousov, I
    Narbonne, V
    Narvskaya, O
    Nastasi, A
    Niobe-Eyangoh, SN
    Pape, JW
    Rasolofo-Razanamparany, V
    Ridell, M
    Rossetti, ML
    Stauffer, F
    Suffys, PN
    Takiff, H
    Texier-Maugein, J
    Vincent, V
    de Waard, JH
    Sola, C
    Rastogi, N
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (05) : 1963 - 1970
  • [10] Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats
    Frothingham, R
    Meeker-O'Connell, WA
    [J]. MICROBIOLOGY-UK, 1998, 144 : 1189 - 1196