RETRACTED: SETDB1 Is Involved in Postembryonic DNA Methylation and Gene Silencing in Drosophila (Retracted article. See vol. 13, art no E0194869, 2018)

被引:20
作者
Gou, Dawei [1 ]
Rubalcava, Monica [1 ]
Sauer, Silvia [1 ]
Mora-Bermudez, Felipe [2 ]
Erdjument-Bromage, Hediye [3 ]
Tempst, Paul [3 ]
Kremmer, Elisabeth [4 ]
Sauer, Frank [1 ,2 ]
机构
[1] Univ Calif Riverside, Dept Biochem, Riverside, CA 92521 USA
[2] Heidelberg Univ, Zentrum Mol Biol, D-6900 Heidelberg, Germany
[3] Mem Sloan Kettering Canc Ctr, Program Mol Biol, New York, NY 10021 USA
[4] German Res Ctr Environm Hlth, Inst Mol Immunol, Helmholtz Zentrum Munchen, Munich, Germany
来源
PLOS ONE | 2010年 / 5卷 / 05期
关键词
CPG-BINDING DOMAIN; EPIGENETIC REGULATION; GENOME; EXPRESSION; TRANSCRIPTION; WIDE; EYE; RBF;
D O I
10.1371/journal.pone.0010581
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
DNA methylation is fundamental for the stability and activity of genomes. Drosophila melanogaster and vertebrates establish a global DNA methylation pattern of their genome during early embryogenesis. Large-scale analyses of DNA methylation patterns have uncovered revealed that DNA methylation patterns are dynamic rather than static and change in a gene-specific fashion during development and in diseased cells. However, the factors and mechanisms involved in dynamic, postembryonic DNA methylation remain unclear. Methylation of lysine 9 in histone H3 (H3-K9) by members of the Su(var)3-9 family of histone methyltransferases (HMTs) triggers embryonic DNA methylation in Arthropods and Chordates. Here, we demonstrate that Drosophila SETDB1 (dSETDB1) can mediate DNA methylation and silencing of genes and retrotransposons. We found that dSETDB1 tri-methylates H3-K9 and binds methylated CpA motifs. Tri-methylation of H3-K9 by dSETDB1 mediates recruitment of DNA methyltransferase 2 (Dnmt2) and Su(var) 205, the Drosophila ortholog of mammalian "Heterochromatin Protein 1'', to target genes for dSETDB1. By enlisting Dnmt2 and Su(var) 205, dSETDB1 triggers DNA methylation and silencing of genes and retrotransposons in Drosophila cells. DSETDB1 is involved in postembryonic DNA methylation and silencing of Rt1b{} retrotransposons and the tumor suppressor gene retinoblastoma family protein 1 (Rb) in imaginal discs. Collectively, our findings implicate dSETDB1 in postembryonic DNA methylation, provide a model for silencing of the tumor suppressor Rb, and uncover a role for cell type-specific DNA methylation in Drosophila development.
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页数:19
相关论文
共 60 条
[1]  
[Anonymous], GENOME BIOL
[2]  
Ashburner M., 2005, Drosophila: A laboratory handbook, V2
[3]   Tetracycline-controlled transcription in eukaryotes: Novel transactivators with graded transactivation potential [J].
Baron, U ;
Gossen, M ;
Bujard, H .
NUCLEIC ACIDS RESEARCH, 1997, 25 (14) :2723-2729
[4]   DNA methylation and gene silencing in cancer [J].
Baylin S.B. .
Nature Clinical Practice Oncology, 2005, 2 (Suppl 1) :S4-S11
[5]   DNA methylation patterns and epigenetic memory [J].
Bird, A .
GENES & DEVELOPMENT, 2002, 16 (01) :6-21
[6]   Methylation-induced repression - Belts, braces, and chromatin [J].
Bird, AP ;
Wolffe, AP .
CELL, 1999, 99 (05) :451-454
[7]   Cell fate determination during G1 phase progression [J].
Blomen, V. A. ;
Boonstra, J. .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2007, 64 (23) :3084-3104
[8]  
BRAND AH, 1993, DEVELOPMENT, V118, P401
[9]   Multiple SET Methyltransferases Are Required to Maintain Normal Heterochromatin Domains in the Genome of Drosophila melanogaster [J].
Brower-Toland, Brent ;
Riddle, Nicole C. ;
Jiang, Hongmei ;
Huisinga, Kathryn L. ;
Elgin, Sarah C. R. .
GENETICS, 2009, 181 (04) :1303-1319
[10]   Gardening the genome:: DNA methylation in Arabidopsis thaliana [J].
Chan, SWL ;
Henderson, IR ;
Jacobsen, SE .
NATURE REVIEWS GENETICS, 2005, 6 (05) :351-360