Cell-specific gene transfer by α-cyclodextrin conjugates with mannosylated polyamidoamine dendrimers

被引：15

作者：

Arima, H ^{[1
]}

Wada, K ^{[1
]}

Kihara, F ^{[1
]}

Tsutsumi, T ^{[1
]}

Hirayama, F ^{[1
]}

Uekama, K ^{[1
]}

机构：

[1] Kumamoto Univ, Fac Pharmaceut Sci, Kumamoto 8620973, Japan

来源：

JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY | 2002年 / 44卷 / 1-4期

关键词：

cyclodextrin; dendrimer; gene transfer; mannose; targeting;

D O I：

10.1023/A:1023092508646

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

To improve the activity and the cell specificity of gene transfer of polyamidoamine starburst dendrimer, we prepared alpha-CyD conjugates with mannosylated dendrimers (generation 2 (G2), man-alpha-CDE conjugates) having various degrees of substitution (DS) of mannose residue. The man-alpha-CDE conjugates (DS 1, 3 and 5) formed complexes with plasmid DNA (pDNA), but man-alpha-CDE conjugate (DS 8) did not. The gene transfer activity of man-alpha-CDE conjugates (DS 1, 3 and 5) and alpha-CDE conjugate was augmented with an increase in the charge ratio of vector/pDNA, without showing cytotoxicity. Man-alpha- CDE conjugates (DS 3 and 5) showed higher gene transfer activity than alpha-CDE conjugate in A549 cells, which recognize mannose, but man-alpha-CDE conjugates (DS 1 and 8) showed almost comparable gene transfer activity to alpha-CDE conjugate (G2). On the other hand, no appreciable enhancing effect of man-alpha-CDE conjugates (DS 3 and 5) on the transfer activity was observed in NIH3T3 cells, which do not recognize mannose. These findings suggest that man-alpha-CDE conjugates (DS 3 and 5) can be new preferable cell-specific non-viral vectors of pDNA to cells which recognize the mannose moiety.

引用

页码：361 / 364

页数：4

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