Shape changes of self-assembled actin bilayer composite membranes

被引:70
作者
Hackl, W [1 ]
Barmann, M [1 ]
Sackmann, E [1 ]
机构
[1] Tech Univ Munich, Biophys Lab, Dept Phys E22, D-85748 Garching, Germany
关键词
D O I
10.1103/PhysRevLett.80.1786
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
We report the self-assembly of thin actin shells beneath the membranes of giant vesicles. Ion-carrier mediated influx of Mg2+ induces actin polymerization in the initially spherical vesicles. Buckling of the vesicles and the formation of blisters after thermally induced bilayer expansion are demonstrated. Bilayer flickering is dominated by tension generated by its coupling to the actin cortex. Quantitative flicker analysis suggests that the bilayer and the actin cortex are separated by 0.4 to 0.5 mu m due to undulation forces.
引用
收藏
页码:1786 / 1789
页数:4
相关论文
共 20 条
[1]  
Alberts B., 2017, MOL BIOL CELL
[2]   Free energies of isolated five- and sevenfold disclinations in hexatic membranes [J].
Deem, MW ;
Nelson, DR .
PHYSICAL REVIEW E, 1996, 53 (03) :2551-2559
[3]   LIPID SWELLING AND LIPOSOME FORMATION MEDIATED BY ELECTRIC-FIELDS [J].
DIMITROV, DS ;
ANGELOVA, MI .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1988, 19 (02) :323-336
[4]   Buckling microtubules in vesicles [J].
Elbaum, M ;
Fygenson, DK ;
Libchaber, A .
PHYSICAL REVIEW LETTERS, 1996, 76 (21) :4078-4081
[5]   NEW PHYSICAL CONCEPTS FOR CELL AMEBOID MOTION [J].
EVANS, E .
BIOPHYSICAL JOURNAL, 1993, 64 (04) :1306-1322
[6]   Energy filtered electron tomography of ice-embedded actin and vesicles [J].
Grimm, R ;
Barmann, M ;
Hackl, W ;
Typke, D ;
Sackmann, E ;
Baumeister, W .
BIOPHYSICAL JOURNAL, 1997, 72 (01) :482-489
[7]   Effects of fully and partially solubilized amphiphiles on bilayer bending stiffness and temperature dependence of the effective tension of giant vesicles [J].
Hackl, W ;
Seifert, U ;
Sackmann, E .
JOURNAL DE PHYSIQUE II, 1997, 7 (08) :1141-1157
[8]  
Kas J, 1996, BIOPHYS J, V70, P609, DOI 10.1016/S0006-3495(96)79630-3
[9]   DIRECT MEASUREMENT OF THE WAVE-VECTOR-DEPENDENT BENDING STIFFNESS OF FREELY FLICKERING ACTIN-FILAMENTS [J].
KAS, J ;
STREY, H ;
BARMANN, M ;
SACKMANN, E .
EUROPHYSICS LETTERS, 1993, 21 (08) :865-870
[10]   SHAPE TRANSITIONS AND SHAPE STABILITY OF GIANT PHOSPHOLIPID-VESICLES IN PURE WATER INDUCED BY AREA-TO-VOLUME CHANGES [J].
KAS, J ;
SACKMANN, E .
BIOPHYSICAL JOURNAL, 1991, 60 (04) :825-844