Selenium supplementation can protect cultured rat cardiomyocytes from hypoxia/reoxygenation damage

被引：14

作者：

Bordoni, A ^{[1
]}

Biagi, PL ^{[1
]}

Angeloni, C ^{[1
]}

Leoncini, E ^{[1
]}

Muccinelli, I ^{[1
]}

Hrelia, S ^{[1
]}

机构：

[1] Univ Bologna, Nutr Res Ctr, Dept Biochem G Moruzzi, I-40126 Bologna, Italy

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2003年 / 51卷 / 06期

关键词：

selenium; hypoxia/reoxygenation; cultured cardiomyocytes; glutathione peroxidase; oxidative stress;

D O I：

10.1021/jf026017d

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The possibility of enhancing glutathione peroxidase (GPx) activity and cytosolic total antioxidant activity (TAA) in normoxia and hypoxia/reoxygenation (H/R) by the supplementation of different concentrations of sodium selenite (SS) or selenomethionine (SM) was investigated in cultured rat cardiomyocytes. To assess the entity of oxidative stress due to H/R, levels of conjugated dienes containing lipids were determined. In normoxia, GPx activity and TAA increased in parallel with the increase in SS and SM supplementation. H/R did not influence GPx activity but lowered TAA; both SS and SM supplementations were effective in increasing GPx activity, the most effective concentration being 1 muM. At this SS and SM concentration, TAA returned to a normoxic value. Conjugated diene production, increased by H/R, was reduced by SS and SM supplementation, the 1 muM concentration appearing to be the most effective one. According to these data Se supplementation represents another possibility to counteract oxidative damage in the myocardium.

引用

页码：1736 / 1740

页数：5

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