Cloning cDNA of RNA-binding proteins by using phage display method: Construction of the phage expression library.

被引：0

作者：

He, YZ ^{[1
]}

Ni, XD ^{[1
]}

Liu, D ^{[1
]}

Qing, GL ^{[1
]}

Li, ZP ^{[1
]}

机构：

[1] CHINESE ACAD SCI,SHANGHAI INST BIOCHEM,SHANGHAI 200031,PEOPLES R CHINA

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 1997年 / 24卷 / 05期

关键词：

phage display; RNA-binding protein; NF-IL6 3'UTR;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to clone cDNAs coding for proteins specially-binding to NF-IL6's 3'UTR, a phagemid expression library was constructed from cDNAs of revertant RR cells. The termination codons of those cloned cDNAs were largely removed by restricted exo III enzyme digestion, in order to facilitate the expression of cDNA as fusion proteins with the phage gene III. Examinations of this library showed that the library includes various lengths of cDNA inserts and it can express exogenous cDNA.

引用

页码：467 / 470

页数：4

共 4 条

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