A novel method for rapid detection of class IIa bacteriocin-producing lactic acid bacteria

被引：42
作者：
Yi, Huaxi [1 ]
Zhang, Lanwei [1 ]
Tuo, Yanfeng [2 ]
Han, Xue [1 ]
Du, Ming [1 ]
机构：
[1] Harbin Inst Technol, Sch Food Sci & Engn, Harbin 150090, Peoples R China
[2] Gansu Agr Univ, Dept Food Sci & Technol, Lanzhou 730070, Peoples R China
来源：
FOOD CONTROL | 2010年 / 21卷 / 04期
关键词：
Class IIa bacteriocin; Lactic acid bacteria; Colony PCR; ANTIMICROBIAL PEPTIDES; QUANTIFICATION; BIOASSAY; NISIN;
D O I：
10.1016/j.foodcont.2009.07.002
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A method based on colony PCR was developed for rapid screening of class IIa bacteriocin-producing lactic acid bacteria. The principle of the method is to detect the presence of bacteriocin gene by using specific degenerate primers to amplify a 3 kb fragment from bacteriocin structural gene to histidine kinase gene regions. Degenerate primers were designed according to the conserved amino acid sequences (YGNGVXCXXXXCXV and LDNAIE) contained in class IIa bacteriocins. This assay showed agreement with the conventional well-diffusion method. These findings indicate that the method proposed in this study is considerably more rapid and sufficient for screening of class IIa bacteriocin-producing bacterial isolates in environmental samples. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：426 / 430
页数：5
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