A novel gas production bioassay of thiosulfate utilizing denitrifying bacteria (TUDB) for the toxicity assessment of heavy metals contaminated water

被引：8

作者：

Ashun, Ebenezer ^{[1
]}

Kang, Woochang ^{[1
]}

Sen Thapa, Bhim ^{[1
]}

Gurung, Anup ^{[1
]}

Rahimnejad, Mostafa ^{[2
]}

Jang, Min ^{[3
]}

Jeon, Byong-Hun ^{[4
]}

Kim, Jung Rae ^{[5
]}

Oh, Sang-Eun ^{[1
]}

机构：

[1] Kangwon Natl Univ, Dept Biol Environm, 192-1 Hyoja-dong, Gangwon Do, Chunchon 200701, South Korea

[2] Babol Noshirvani Univ Technol, Biofuel & Renewable Energy Res Ctr, Chem Engn Dept, Babol, Iran

[3] Kwangwoon Univ, Dept Environm Engn, 20 Kwangwoon Ro, Seoul 01897, South Korea

[4] Hanyang Univ, Dept Earth Resources & Environm Engn, Seoul 04763, South Korea

[5] Pusan Natl Univ, Sch Chem Engn, 63 Busandeahak Ro, Pusan 46241, South Korea

来源：

CHEMOSPHERE | 2022年 / 303卷

关键词：

Autotrophic denitrification; Thiosulfate; Bioassay; Gas production; Heavy metals; Water toxicity; SULFUR-OXIDIZING BACTERIA; AUTOTROPHIC DENITRIFICATION; MICROBIAL COMMUNITIES; GROWTH-RATE; NITRATE; BIOSENSOR; RESPIRATION; INHIBITION; REACTORS; NITROGEN;

D O I：

10.1016/j.chemosphere.2022.134902

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study reports for the first-time the possibility of deploying gas production by thiosulfate utilizing denitrifying bacteria (TUDB) as a proxy to evaluate water toxicity. The test relies on gas production by TUDB due to inhibited metabolic activity in the presence of toxicants. Gas production was measured using a bubble-type respirometer. Optimization studies indicated that 300 mg NO3- - N/L, 0.5 mL acclimated culture, and 2100 mg S2O32- /L were the ideal conditions facilitating the necessary volume of gas production for sensitive data generation. Determined EC50 values of the selected heavy metals were: Cr6+, 0.51 mg/L; Ag+, 2.90 mg/L; Cu2+, 2.90 mg/L; Ni2+, 3.60 mg/L; As3+, 4.10 mg/L; Cd2+, 5.56 mg/L; Hg2+, 8.06 mg/L; and Pb2+, 19.3 mg/L. The advantages of this method include operational simplicity through the elimination of cumbersome preprocessing procedures which are used to eliminate interferences caused by turbidity when the toxicity of turbid samples is determined via spectrophotometry.

引用

页数：11

共 56 条

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