Bacterial cellulose-lactoferrin as an antimicrobial edible packaging

被引：104

作者：

Padrao, Jorge ^{[1
]}

Goncalves, Sara ^{[1
]}

Silva, Joao P. ^{[1
]}

Sencadas, Vitor ^{[2
,3
]}

Lanceros-Mendez, Senentxu ^{[2
]}

Pinheiro, Ana C. ^{[1
]}

Vicente, Antonio A. ^{[1
]}

Rodrigues, Ligia R. ^{[1
]}

Dourado, Fernando ^{[1
]}

机构：

[1] Univ Minho, Ctr Biol Engn, Campus Gualtar, P-4710057 Braga, Portugal

[2] Univ Minho, Ctr Dept Phys, Campus Gualtar, P-4710057 Braga, Portugal

[3] Univ Wollongong, Sch Mech Mat & Mechatron Engn, Wollongong, NSW 2522, Australia

来源：

FOOD HYDROCOLLOIDS | 2016年 / 58卷

关键词：

Bacterial cellulose; Bovine lactoferrin; Active packaging; Antimicrobial; Cytotoxicity; Gastrointestinal tract model; WATER-VAPOR PERMEABILITY; PHYSICOCHEMICAL PROPERTIES; LISTERIA-MONOCYTOGENES; MECHANICAL-PROPERTIES; BOVINE LACTOFERRIN; BINDING; FILMS; STABILITY; MEAT; METHYLCELLULOSE;

D O I：

10.1016/j.foodhyd.2016.02.019

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Bacterial cellulose (BC) films from two distinct sources (obtained by static culture with Gluconacetobacter xylinus ATCC 53582 (BC1) and from a commercial source (BC2)) were modified by bovine lactoferrin (bLF) adsorption. The functionalized films (BC + bLF) were assessed as edible antimicrobial packaging, for use in direct contact with highly perishable foods, specifically fresh sausage as a model of meat products. BC + bLF films and sausage casings were characterized regarding their water vapour permeability (WVP), mechanical properties, and bactericidal efficiency against two food pathogens, Escherichia coli and Staphylococcus aureus. Considering their edibility, an in vitro gastrointestinal tract model was used to study the changes occurring in the BC films during passage through the gastrointestinal tract. Moreover, the cytotoxicity of the BC films against 3T3 mouse embryo fibroblasts was evaluated. BC1 and BC2 showed equivalent density, WVP and maximum tensile strength. The percentage of bactericidal efficiency of BC1 and BC2 with adsorbed bLF (BC1 + bLF and BC2 + bLF, respectively) in the standalone films and in inoculated fresh sausages, was similar against E. coli (mean reduction 69% in the films per se versus 94% in the sausages) and S. aureus (mean reduction 97% in the films per se versus 36% in the case sausages). Moreover, the BC1 + bLF and BC2 + bLF films significantly hindered the specific growth rate of both bacteria. Finally, no relevant cytotoxicity against 3T3 fibroblasts was found for the films before and after the simulated digestion. BC films with adsorbed bLF may constitute an approach in the development of bio-based edible antimicrobial packaging systems. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：126 / 140

页数：15

共 77 条

[41]

Lacroix M, 2009, EDIBLE FILMS AND COATINGS FOR FOOD APPLICATIONS, P347, DOI 10.1007/978-0-387-92824-1_13

[42] Adding enzymatically modified gelatin to enhance the rehydration abilities and mechanical properties of bacterial cellulose [J].

Lin, Shih-Bin ;

Hsu, Chieh-Ping ;

Chen, Li-Chen ;

Chen, Hui-Huang .

FOOD HYDROCOLLOIDS, 2009, 23 (08) :2195-2203

[43]

Chambi HNM, 2011, CIENCIA TECNOL ALIME, V31, P739, DOI 10.1590/S0101-20612011000300029

[44]

Maneesri J., 2012, Journal of Agricultural Science and Technology A, V2, P86

[45] Influence of α-tocopherol on physicochemical properties of chitosan-based films [J].

Martins, Joana T. ;

Cerqueira, Miguel A. ;

Vicente, Antonio A. .

FOOD HYDROCOLLOIDS, 2012, 27 (01) :220-227

[46] Electrically conducting nanocomposites: preparation and properties of polyaniline (PAni)-coated bacterial cellulose nanofibers (BC) [J].

Mueller, D. ;

Mandelli, J. S. ;

Marins, J. A. ;

Soares, B. G. ;

Porto, L. M. ;

Rambo, C. R. ;

Barra, G. M. O. .

CELLULOSE, 2012, 19 (05) :1645-1654

[47] Potential of a nisin-containing bacterial cellulose film to inhibit Listeria monocytogenes on processed meats [J].

Nguyen, V. T. ;

Gidley, M. J. ;

Dykes, G. A. .

FOOD MICROBIOLOGY, 2008, 25 (03) :471-478

[48] PERMEABILITY AND MECHANICAL-PROPERTIES OF CELLULOSE-BASED EDIBLE FILMS [J].

PARK, HJ ;

WELLER, CL ;

VERGANO, PJ ;

TESTIN, RF .

JOURNAL OF FOOD SCIENCE, 1993, 58 (06) :1361-1364

[49] Active and Intelligent Packaging for the Food Industry [J].

Pereira de Abreu, D. A. ;

Cruz, J. M. ;

Paseiro Losada, P. .

FOOD REVIEWS INTERNATIONAL, 2012, 28 (02) :146-187

[50]

Perez-Perez C., 2006, Advances in agricultural and food biotechnology, P193

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