Hexokinase PII has a double cytosolic-nuclear localisation in Saccharomyces cerevisiae

被引：67

作者：

Randez-Gil, F

Herrero, P

Sanz, P

Prieto, JA

Moreno, F ^{[1
]}

机构：

[1] Univ Oviedo, Inst Biotecnol Asturias, Dept Bioquim & Biol Mol, E-33006 Oviedo, Spain

[2] CSIC, Inst Agroquim & Tecnol Alimentos, Dept Biotecnol Alimentos, Burjassot 46100, Spain

来源：

FEBS LETTERS | 1998年 / 425卷 / 03期

关键词：

Saccharomyces cerevisiae; HXK2; nucleus;

D O I：

10.1016/S0014-5793(98)00289-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here that the HXK2 gene product, isoenzyme Pn of hexokinase, is located in both the nucleus and the cytoplasm of Saccharomyces cerevisiae cells. This conclusion is supported by assays of hexokinase-specific activity in isolated nuclei from wild-type and hxk1lhxk2 double mutant strains, by immunoblot experiments using anti-Hxk2 antibodies and by observation of the fluorescence distribution of a Hsk2-GFP fusion protein in cells transformed with the HXK2::gfp gene. (C) 1998 Federation of European Biochemical Societies.

引用

页码：475 / 478

页数：4

共 36 条

[11] GENETIC-EVIDENCE FOR A ROLE OF HEXOKINASE ISOENZYME PII IN CARBON CATABOLITE REPRESSION IN SACCHAROMYCES-CEREVISIAE
ENTIAN, KD
MECKE, D
JOURNAL OF BIOLOGICAL CHEMISTRY, 1982, 257 (02) : 870 - 874
[12] A CARBON CATABOLITE REPRESSION MUTANT OF SACCHAROMYCES-CEREVISIAE WITH ELEVATED HEXOKINASE-ACTIVITY - EVIDENCE FOR REGULATORY CONTROL OF HEXOKINASE PII SYNTHESIS
ENTIAN, KD
MOLECULAR & GENERAL GENETICS, 1981, 184 (02): : 278 - 282
[13] Physiological properties of Saccharomyces cerevisiae from which hexokinase II has been deleted
Diderich, JA
Raamsdonk, LM
Kruckeberg, AL
Berden, JA
Van Dam, K
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (04) : 1587 - 1593
[14] CLONING OF HEXOKINASE ISOENZYME PI FROM SACCHAROMYCES-CEREVISIAE - PI TRANSFORMANTS CONFIRM THE UNIQUE ROLE OF HEXOKINASE ISOENZYME PII FOR GLUCOSE REPRESSION IN YEASTS
ENTIAN, KD
KOPETZKI, E
FROHLICH, KU
MECKE, D
MOLECULAR & GENERAL GENETICS, 1984, 198 (01): : 50 - 54
[15] SACCHAROMYCES-CEREVISIAE MUTANTS PROVIDE EVIDENCE OF HEXOKINASE PII AS A BIFUNCTIONAL ENZYME WITH CATALYTIC AND REGULATORY DOMAINS FOR TRIGGERING CARBON CATABOLITE REPRESSION
ENTIAN, KD
FROHLICH, KU
JOURNAL OF BACTERIOLOGY, 1984, 158 (01) : 29 - 35
[16] Characterisation of a tripartite nuclear localisation sequence in the regulatory protein Lys14 of Saccharomyces cerevisiae
El Alami, M
Feller, A
Piérard, A
Dubois, E
CURRENT GENETICS, 2000, 38 (02) : 78 - 86
[17] Characterisation of a tripartite nuclear localisation sequence in the regulatory protein Lys14 of Saccharomyces cerevisiae
Mohamed El Alami
André Feller
André Piérard
Evelyne Dubois
Current Genetics, 2000, 38 : 78 - 86
[18] ISOLATION AND CHARACTERIZATION OF CATALYTICALLY-ACTIVE HEXOKINASE PII MUTANTS WITH PRECIPITATED GLUCOSE REPRESSION - A MODEL FOR A BIFUNCTIONAL ENZYME IN THE YEAST SACCHAROMYCES-CEREVISIAE
ENTIAN, KD
FROHLICH, KU
MECKE, D
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1983, 364 (04): : 329 - 329
[19] TRANSFORMATION OF A GLUCOSE NEGATIVE MUTANT OF PACHYSOLEN-TANNOPHILUS WITH A PLASMID CARRYING THE CLONED HEXOKINASE-PII GENE FROM SACCHAROMYCES-CEREVISIAE
WEDLOCK, DN
THORNTON, RJ
BIOTECHNOLOGY LETTERS, 1989, 11 (09) : 601 - 604
[20] The glucose-regulated nuclear localization of hexokinase 2 in Saccharomyces cerevisiae is Mig1-dependent
Ahuatzi, D
Herrero, P
de la Cera, T
Moreno, F
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (14) : 14440 - 14446

← 1 2 3 4 →