Hexokinase PII has a double cytosolic-nuclear localisation in Saccharomyces cerevisiae

被引：67
|
作者：
Randez-Gil, F
Herrero, P
Sanz, P
Prieto, JA
Moreno, F [1 ]
机构：
[1] Univ Oviedo, Inst Biotecnol Asturias, Dept Bioquim & Biol Mol, E-33006 Oviedo, Spain
[2] CSIC, Inst Agroquim & Tecnol Alimentos, Dept Biotecnol Alimentos, Burjassot 46100, Spain
来源：
FEBS LETTERS | 1998年 / 425卷 / 03期
关键词：
Saccharomyces cerevisiae; HXK2; nucleus;
D O I：
10.1016/S0014-5793(98)00289-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe here that the HXK2 gene product, isoenzyme Pn of hexokinase, is located in both the nucleus and the cytoplasm of Saccharomyces cerevisiae cells. This conclusion is supported by assays of hexokinase-specific activity in isolated nuclei from wild-type and hxk1lhxk2 double mutant strains, by immunoblot experiments using anti-Hxk2 antibodies and by observation of the fluorescence distribution of a Hsk2-GFP fusion protein in cells transformed with the HXK2::gfp gene. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码：475 / 478
页数：4
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