Characterization of Fertile Homozygous Genotypes from Anther Culture in Apple

被引：8

作者：

Okada, Hatsuhiko ^{[1
]}

Ohashi, Yoshitaka ^{[1
]}

Sato, Mamoru ^{[1
]}

Matsuno, Hideyuki ^{[2
]}

Yamamoto, Toshiya ^{[3
]}

Kim, Hoytaek ^{[3
]}

Tukuni, Tatsuro ^{[4
]}

Komori, Sadao ^{[5
]}

机构：

[1] Fukushima Agr Technol Ctr, Fruit Tree Res Ctr, Fukushima 9600231, Japan

[2] Fukushima Agr Technol Ctr, Coll Agr, Fukushima 9690292, Japan

[3] Natl Inst Fruit Tree Sci, Tsukuba, Ibaraki 3058605, Japan

[4] Chiba Prefectural Agr & Forestry Res Ctr, Midori Ku, Chiba 2660006, Japan

[5] Iwate Univ, Fac Agr, Dept Agrobiosci, Morioka, Iwate 0208550, Japan

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE | 2009年 / 134卷 / 06期

关键词：

androgenesis; doubled haploids; flow cytometry; Malus xdomestica; morphology; SSR analysis; S-allele; IN-VITRO ANDROGENESIS; MOLECULAR CHARACTERIZATION; S-ALLELE; MICROSATELLITES; INDUCTION; CULTIVARS; MARKERS; FRUITS;

D O I：

10.21273/JASHS.134.6.641

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Doubled haploids can improve the efficiency of breeding and genetic study in apple (Mains xdomestica Borkh.). Seventeen homozygous genotypes were obtained by in vitro anther culture from 'Senshu' apple. Flow cytometry analysis revealed that the ploidy level of the anther-derived plantlets was diploid. Simple sequence repeat (SSR) analysis determined the origin and homozygous status of the anther-derived plantlets. The results of S-RNase polymerase chain reaction (PCR)-digestion analysis reinforced the homozygous state. The morphological characteristics and reproductive potential of the doubled haploids were investigated. It is especially interesting that one of the doubled haploids derived from anther culture had fertile pollen grains (rate of in vitro germination was 20.6%-33.0%), and several progenies were obtained from the cross between 'Prima' apple and the doubled haploid. The hybridism of these seedlings was confirmed by SSR analysis. Furthermore, the female gametes of the doubled haploid showed slight fertility. Thus, this doubled haploid will be valuable for breeding and genetic studies.

引用

页码：641 / 648

页数：8

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