TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade

被引：224

作者：

Koike, Chieko ^{[1
]}

Obara, Takehisa ^{[3
]}

Uriu, Yoshitsugu ^{[4
]}

Numata, Tomohiro ^{[4
]}

Sanuki, Rikako ^{[1
]}

Miyata, Kentarou ^{[5
]}

Koyasu, Toshiyuki ^{[5
]}

Ueno, Shinji ^{[5
]}

Funabiki, Kazuo ^{[2
]}

Tani, Akiko ^{[1
]}

Ueda, Hiroshi ^{[6
]}

Kondo, Mineo ^{[5
]}

Mori, Yasuo ^{[4
]}

Tachibana, Masao ^{[3
]}

Furukawa, Takahisa ^{[1
]}

机构：

[1] Osaka Biosci Inst, Dept Dev Biol, Osaka 5650874, Japan

[2] Osaka Biosci Inst, Dept Syst Biol, Osaka 5650874, Japan

[3] Univ Tokyo, Grad Sch Humanities & Sociol, Dept Psychol, Tokyo 1130033, Japan

[4] Kyoto Univ, Grad Sch Engn, Dept Synthet Chem & Biol Chem, Kyoto 6158510, Japan

[5] Nagoya Univ, Grad Sch Med, Dept Ophthalmol, Nagoya, Aichi 4668550, Japan

[6] Gifu Univ, Fac Engn, Dept Biomol Sci, Gifu 5011193, Japan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2010年 / 107卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

G protein; retina; photoresponse; glutamate; vision; METABOTROPIC GLUTAMATE-RECEPTOR; MOUSE RETINA; SYNAPTIC-TRANSMISSION; NIGHT BLINDNESS; ALPHA-SUBUNIT; G-PROTEIN; GENE; ROD; EXPRESSION; MELASTATIN;

D O I：

10.1073/pnas.0912730107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An essential step in intricate visual processing is the segregation of visual signals into ON and OFF pathways by retinal bipolar cells (BCs). Glutamate released from photoreceptors modulates the photoresponse of ON BCs via metabotropic glutamate receptor 6 (mGluR6) and G protein (Go) that regulates a cation channel. However, the cation channel has not yet been unequivocally identified. Here, we report a mouse TRPM1 long form (TRPM1-L) as the cation channel. We found that TRPM1-L localization is developmentally restricted to the dendritic tips of ON BCs in colocalization with mGluR6. TRPM1 null mutant mice completely lose the photoresponse of ON BCs but not that of OFF BCs. In the TRPM1-L-expressing cells, TRPM1-L functions as a constitutively active nonselective cation channel and its activity is negatively regulated by Go in the mGluR6 cascade. These results demonstrate that TRPM1-L is a component of the ON BC transduction channel downstream of mGluR6 in ON BCs.

引用

页码：332 / 337

页数：6

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