Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis

被引：13

作者：

Aguiar, Juliana Martins ^{[1
]}

Silva, Sheila Oliveira ^{[1
]}

dos Santos, Valdir Azevedo ^{[2
]}

Taniwaki, Sueli Akemi ^{[1
]}

Ferreira de Sousa Oliveira, Tricia Maria ^{[3
]}

Ferreira, Helena Lage ^{[3
]}

Keid, Lara Borges ^{[3
]}

Gregori, Fabio ^{[1
]}

Soares, Rodrigo Martins ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecnia, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP, Brazil

[2] Univ Sao Paulo, Dept Farm, Lab Clin Hosp Univ, BR-09500900 Sao Paulo, SP, Brazil

[3] Univ Sao Paulo, Dept Vet Med, Fac Zootecnia & Engn Alimentos, BR-09500900 Sao Paulo, SP, Brazil

来源：

REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA | 2016年 / 25卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

Giardia duodenalis; micromanipulation; heterozygosity; genetic recombination; cysts; MOLECULAR EPIDEMIOLOGY; SEQUENCE-ANALYSIS; IDENTIFICATION; PHYLOGENIES; GENE; DNA;

D O I：

10.1590/S1984-29612016031

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Giardia duodenalis is divided into eight assemblages (named A to H). Isolates of assemblage A are divided into four sub-assemblages (AI, AII, AIII and AIV). While isolates of sub-assemblage AII are almost exclusively detected in human hosts, isolates of assemblage B are encountered in a multitude of animal hosts and humans. Here, we isolated single cysts of G. duodenalis from a human stool sample and found that one of them had overlaps of assemblage AII and B alleles and an unexpectedly high number of variants of the beta-giardin (Bg) and GLORF-C4 (OrfC4) alleles. In addition, one of the Bg alleles of that cyst had a fragment of sub-assemblage AII interspersed with fragments of assemblage B, thus indicating that this allele may be a recombinant between sequences A and B. Our results are unprecedented and put a check on the statement that different assemblages of G. duodenalis represent species with different host specificities.

引用

页码：187 / 195

页数：9

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