E4BP4 is a cardiac survival factor and essential for embryonic heart development

被引：18

作者：

Weng, Yi-Jiun ^{[1
]}

Hsieh, Dennis Jine-Yuan ^{[2
]}

Kuo, Wei-Wen ^{[3
]}

Lai, Tung-Yuan ^{[4
]}

Hsu, Hsi-Hsien ^{[5
,6
]}

Tsai, Chang-Hai ^{[7
]}

Tsai, Fuu-Jen ^{[8
]}

Lin, Ding-Yu ^{[9
]}

Lin, James A. ^{[9
]}

Huang, Chih-Yang ^{[8
,9
,10
]}

Tung, Kwong-Chung ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Dept Vet Med, Taichung 402, Taiwan

[2] Chung Shan Med Univ, Sch Med Lab & Biotechnol, Taichung 402, Taiwan

[3] China Med Univ, Dept Biol Sci & Technol, Taichung 404, Taiwan

[4] China Med Univ, Sch Postbaccalaureate Chinese Med, Taichung 404, Taiwan

[5] Mackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan

[6] Mackay Med Nursing & Management Coll, Taipei, Taiwan

[7] Asia Univ, Dept Healthcare Adm, Taichung, Taiwan

[8] China Med Univ, Grad Inst Chinese Med Sci, Taichung 404, Taiwan

[9] China Med Univ, Grad Inst Basic Med Sci, Taichung 404, Taiwan

[10] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung, Taiwan

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2010年 / 340卷 / 1-2期

关键词：

E4BP4; Heart development; Cardiac survival factor; TRANSCRIPTIONAL REPRESSION; HEMATOPOIETIC-CELLS; BZIP FAMILY; MYOCARDIAL-INFARCTION; GENE-EXPRESSION; FACTOR-II; ZEBRAFISH; MODEL; TOXICITY; GROWTH;

D O I：

10.1007/s11010-010-0417-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The bZIP transcription factor E4BP4, has been demonstrated to be a survival factor in pro-B lymphocytes. GATA factors play important roles in transducing the IL-3 survival signal and transactivating the downstream survival gene, E4BP4. In heart, GATA sites are essential for proper transcription of several cardiac genes, and GATA-4 is a mediator of cardiomyocyte survival. However, the role E4BP4 plays in heart is still poorly understood. In this study, Dot-blot hybridization assays using Dig-labeled RNA probes revealed that the E4BP4 gene was expressed in cardiac tissue from several species including, monkey, dog, rabbit, and human. Western blot analysis showed that the E4BP4 protein was consistently present in all of these four species. Furthermore, immunohistochemistry revealed that the E4BP4 protein was overexpressed in diseased heart tissue in comparison with normal heart tissue. In addition, the overexpression of E4BP4 in vitro activated cell survival signaling pathway of cardiomyocytes. At last, siRNA-mediated knock down of E4BP4 in zebrafish resulted in malformed looping of the embryonic heart tube and decreased heart beating. Based on these results, we conclude that E4BP4 plays as a survival factor in heart and E4BP4 is essential for proper embryonic heart development.

引用

页码：187 / 194

页数：8

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