Production of a thermostable alkali-tolerant xylanase from Bacillus circulans AB 16 grown on wheat straw

被引：32

作者：

Dhillon, A ^{[1
]}

Khanna, S ^{[1
]}

机构：

[1] Tata Energy Res Inst, India Habitat Ctr, New Delhi 110003, India

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2000年 / 16卷 / 04期

关键词：

Bacillus circulans; pulping; thermostability; wheat straw; xylanase;

D O I：

10.1023/A:1008911026606

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacillus circulans AB 16 was able to produce 50 IU/ml of xylanase, with negligible cellulase activity when grown on untreated wheat straw. The pH optimum of the crude enzyme was 6-7 with a temperature optimum of 80 degrees C. The enzyme showed high pH and thermal stability retaining 100% activity at 60 degrees C, pH 8 and 9 after 2.5 h of incubation. The residual activity at 70 degrees C after 2.5 h was 62% and 45% at pH 8 and 9, respectively. At 75 degrees C only 22.2% activity remained at pH 8 after 1 h incubation. Since Kraft pulp is alkaline this enzyme could be used for prebleaching of pulp at temperatures up to 70 degrees C without pH adjustment.

引用

页码：325 / 327

页数：3