Extra virgin olive oil phenolic extracts counteract the pro-oxidant effect of dietary oxidized lipids in human intestinal cells

被引：56

作者：

Incani, Alessandra ^{[1
]}

Serra, Gessica ^{[1
]}

Atzeri, Angela ^{[1
]}

Melis, Maria Paola ^{[1
]}

Serreli, Gabriele ^{[1
]}

Bandino, Giovanni ^{[2
]}

Sedda, Piergiorgio ^{[2
]}

Campus, Marco ^{[2
]}

Tuberoso, Carlo I. G. ^{[3
]}

Deiana, Monica ^{[1
]}

机构：

[1] Univ Cagliari, Dept Biomed Sci, Cagliari, Italy

[2] Agr Res Agcy Sardinia, AGRIS Sardinia, Sassari, Italy

[3] Univ Cagliari, Dept Life & Environm Sci, Cagliari, Italy

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2016年 / 90卷

关键词：

Olive oil; Oxidative stress; Caco-2; cells; Oxysterols; Phenolic extract; COLON-CANCER CELLS; OXIDATIVE STRESS; GLUTATHIONE SYNTHESIS; WINE EXTRACTS; CACO-2; POLYPHENOLS; OXYSTEROLS; APOPTOSIS; DAMAGE; METABOLISM;

D O I：

10.1016/j.fct.2016.02.015

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The phenolic fraction of extra virgin olive oil (EVOO) concentrates before absorption in the intestinal lumen, where it may contribute to the modulation of enterocytes response to oxidative and inflammatory stimuli. We evaluated the ability of two monovarietal EVOOs phenolic extracts, Bosana and Nera di Gonnos/Tonda di Cagliari, typical and widespread varieties in Sardinia (Italy), to counteract in enterocytes like Caco-2 cells the pro-oxidant action of oxidized lipids, tert-butyl hydroperoxide (TBH) or a mixture of oxysterols of dietary origin. We confirmed that TBH treatment causes a significant increase of ROS production, GSH depletion, increase of MDA, fatty acids hydroperoxides and 7-ketocholesterol, and showed first evidence of oxidative imbalance and cell damage due to oxysterols exposure. Pre incubation of cells with the phenolic extracts significantly attenuated oxidative modifications. Bosana extract showed the highest concentration of total phenols, mainly hydroxytyrosol and tyrosol, and was the most active in presence of TBH, where the free radical scavenging activity of these simple phenols seems to be a determining factor. The two extracts were equally effective, in spite of the different composition, in presence of oxysterols, where ROS production probably occurs according to different and more complex mechanisms. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：171 / 180

页数：10

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[1] OXIDATIVE STRESS: AN ESSENTIAL FACTOR IN THE PATHOGENESIS OF GASTROINTESTINAL MUCOSAL DISEASES [J].