Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction

被引：8

作者：

Soga, Keisuke ^{[1
]}

Nakamura, Kosuke ^{[1
]}

Ishigaki, Takumi ^{[1
]}

Kimata, Shinya ^{[1
]}

Ohmori, Kiyomi ^{[2
]}

Kishine, Masahiro ^{[3
]}

Mano, Junichi ^{[3
]}

Takabatake, Reona ^{[3
]}

Kitta, Kazumi ^{[3
]}

Nagoya, Hiroyuki ^{[4
]}

Kondo, Kazunari ^{[1
]}

机构：

[1] Natl Inst Hlth Sci, Kawasaki Ku, 3-25-26 Tonomachi, Kawasaki, Kanagawa 2159501, Japan

[2] Kanagawa Prefectural Inst Publ Hlth, Chem Div, 1-3-1 Shimomachiya, Chigasaki, Kanagawa 2530087, Japan

[3] Natl Agr & Food Res Org, Food Res Inst, Div Analyt Sci, 2-1-12 Kannondai, Tsukuba, Ibaraki 3058642, Japan

[4] Fisheries Res & Educ Agcy, Natl Res Inst Aquaculture, Res Ctr Aquat Breeding, 224-1 Hiruta, Tamaki, Mie 5190423, Japan

来源：

FOOD CHEMISTRY | 2020年 / 305卷

关键词：

Genetically modified; Salmon; AquAdvantage; Detection method; Real-time polymerase chain reaction; Salmo salar; GROWTH-HORMONE GENE; FOOD;

D O I：

10.1016/j.foodchem.2019.125426

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Genetically modified (GM) Atlantic salmon, AquAdvantage (AquAd), was the first GM animal approved officially for human consumption. Many countries monitor the use of this product under their GM regulations, but a pragmatic system for AquAd-specific detection is needed. Here, we developed a real-time polymerase chain reaction method with high sensitivity for detection of AquAd in foods. This method showed high specificity for the AquAd transgene and the detection limit was 12.5-25 targeted DNA copies per test reaction. An inter-laboratory study using the method developed demonstrated reproducibility at > 0.1% (w/w) AquAd content.

引用

页数：6

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