Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction

被引：8
|
作者：
Soga, Keisuke [1 ]
Nakamura, Kosuke [1 ]
Ishigaki, Takumi [1 ]
Kimata, Shinya [1 ]
Ohmori, Kiyomi [2 ]
Kishine, Masahiro [3 ]
Mano, Junichi [3 ]
Takabatake, Reona [3 ]
Kitta, Kazumi [3 ]
Nagoya, Hiroyuki [4 ]
Kondo, Kazunari [1 ]
机构：
[1] Natl Inst Hlth Sci, Kawasaki Ku, 3-25-26 Tonomachi, Kawasaki, Kanagawa 2159501, Japan
[2] Kanagawa Prefectural Inst Publ Hlth, Chem Div, 1-3-1 Shimomachiya, Chigasaki, Kanagawa 2530087, Japan
[3] Natl Agr & Food Res Org, Food Res Inst, Div Analyt Sci, 2-1-12 Kannondai, Tsukuba, Ibaraki 3058642, Japan
[4] Fisheries Res & Educ Agcy, Natl Res Inst Aquaculture, Res Ctr Aquat Breeding, 224-1 Hiruta, Tamaki, Mie 5190423, Japan
来源：
FOOD CHEMISTRY | 2020年 / 305卷
关键词：
Genetically modified; Salmon; AquAdvantage; Detection method; Real-time polymerase chain reaction; Salmo salar; GROWTH-HORMONE GENE; FOOD;
D O I：
10.1016/j.foodchem.2019.125426
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Genetically modified (GM) Atlantic salmon, AquAdvantage (AquAd), was the first GM animal approved officially for human consumption. Many countries monitor the use of this product under their GM regulations, but a pragmatic system for AquAd-specific detection is needed. Here, we developed a real-time polymerase chain reaction method with high sensitivity for detection of AquAd in foods. This method showed high specificity for the AquAd transgene and the detection limit was 12.5-25 targeted DNA copies per test reaction. An inter-laboratory study using the method developed demonstrated reproducibility at > 0.1% (w/w) AquAd content.
引用
收藏
页数：6
相关论文
共 50 条
  • [21] Development and application of a real-time polymerase chain reaction method for Campylobacter jejuni detection
    Mao-Jun Zhang
    Bo Qiao
    Xue-Bin Xu
    Jian-Zhong Zhang
    World Journal of Gastroenterology, 2013, (20) : 3090 - 3095
  • [22] Inter laboratory Validation Study of an Event-Specific Real-time Polymerase Chain Reaction Detection Method for Genetically Modified 55-1 Papaya
    Noguchi, Akio
    Nakamura, Kosuke
    Sakata, Kozue
    Kobayashi, Tomoko
    Akiyama, Hiroshi
    Kondo, Kazunari
    Teshima, Reiko
    Ohmori, Kiyomi
    Kasahara, Masaki
    Takabatake, Reona
    Kitta, Kazumi
    JOURNAL OF AOAC INTERNATIONAL, 2013, 96 (05) : 1054 - 1058
  • [23] Identification of chickpea (Cicer arietinum) in foods using a novel real-time polymerase chain reaction detection method
    Nakamura, Kosuke
    Ishigaki, Takumi
    Kobayashi, Tomoko
    Kimata, Shinya
    Soga, Keisuke
    Fujii, Uki
    Kishine, Masahiro
    Takabatake, Reona
    Mano, Junichi
    Kitta, Kazumi
    Kawakami, Hiroshi
    Nishimaki-Mogami, Tomoko
    Kondo, Kazunari
    JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2018, 71 : 8 - 16
  • [24] Detection of carious microorganisms using real-time Polymerase Chain Reaction
    Martin, FE
    Nadkarni, M
    Hunter, N
    JOURNAL OF DENTAL RESEARCH, 2001, 80 (04) : 979 - 979
  • [25] Detection and differentiation of leptospires using real-time polymerase chain reaction
    Malathi, G.
    Tirumurugaan, K. G.
    Vijayarani, K.
    Kumanan, K.
    INDIAN JOURNAL OF ANIMAL SCIENCES, 2010, 80 (04): : 279 - 283
  • [26] Interlaboratory Validation of an Event-Specific Real Time Polymerase Chain Reaction Detection Method for Genetically Modified DAS59132 Maize
    Akiyama, Hiroshi
    Sakata, Kozue
    Spiegelhalter, Frank
    Furui, Satoshi
    Nakashima, Akie
    Kitta, Kazumi
    Teshima, Reiko
    FOOD HYGIENE AND SAFETY SCIENCE, 2010, 51 (02): : 65 - 70
  • [27] Detection Method for Genetically Modified Carnation Plants Using Multiplex Polymerase Chain Reaction
    Kim, Jae-Hwan
    Lee, Jea-Eun
    Yoo, Sang-Jin
    Park, Young-Doo
    Kim, Hae-Yeong
    KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY, 2010, 28 (02) : 275 - 280
  • [28] Adenovirus of psittacine birds:: investigations on isolation and development of a real-time polymerase chain reaction for specific detection
    Lueschow, D.
    Prusas, C.
    Lierz, M.
    Gerlach, H.
    Soike, D.
    Hafez, H. M.
    AVIAN PATHOLOGY, 2007, 36 (06) : 487 - 494
  • [29] Detection of genetically modified soybeans and maize by the polymerase chain reaction method
    Lin, HY
    Chiueh, LC
    Shih, DYC
    JOURNAL OF FOOD AND DRUG ANALYSIS, 2000, 8 (03) : 200 - 207
  • [30] Development of a novel real-time polymerase chain reaction assay for the detection of Helicobacter pylori in wastewater samples
    Piqueres, P
    Moreno, Y
    Alonso, JL
    Botella, S
    Hernández, J
    Ferrús, MA
    HELICOBACTER, 2005, 10 (05) : 521 - 521
12345