The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens

被引：22

作者：

Psifidi, A. ^{[1
,2
]}

Fife, M. ^{[3
]}

Howell, J. ^{[3
]}

Matika, O. ^{[1
,2
]}

van Diemen, P. M. ^{[4
]}

Kuo, R. ^{[1
,2
]}

Smith, J. ^{[1
,2
]}

Hocking, P. M. ^{[1
,2
]}

Salmon, N. ^{[3
]}

Jones, M. A. ^{[5
]}

Hume, D. A. ^{[1
,2
]}

Banos, G. ^{[6
]}

Stevens, M. P. ^{[1
,2
]}

Kaiser, P. ^{[1
,2
]}

机构：

[1] Univ Edinburgh, Roslin Inst, Easter Bush EH25 9RG, Midlothian, Scotland

[2] Univ Edinburgh, Royal Dick Sch Vet Studies, Easter Bush EH25 9RG, Midlothian, Scotland

[3] Pirbright Inst, Genet & Genom Grp, Woking GU24 0NF, Surrey, England

[4] Ctr Cellular & Mol Physiol, Nuffield Dept Clin Med, Jenner Inst, Roosevelt Dr, Oxford OX3 7BN, England

[5] Univ Nottingham, Sch Vet Med & Sci, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England

[6] Scotlands Rural Coll, Easter Bush EH25 9RG, Midlothian, Scotland

来源：

BMC GENOMICS | 2016年 / 17卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

Campylobacter; Chicken; Resistance; Back-cross; Advanced intercross; Genome-wide association; Quantitative trait; QUANTITATIVE TRAIT LOCI; MAJOR HISTOCOMPATIBILITY COMPLEX; SALMONELLA CARRIER-STATE; 2 GENETIC LINES; WIDE ASSOCIATION; EPITHELIAL-CELLS; IMMUNE-RESPONSE; MATERNAL ANTIBODIES; BODY-WEIGHT; B-COMPLEX;

D O I：

10.1186/s12864-016-2612-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. In the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to Campylobacter could potentially reduce its subsequent entry into the food chain. Campylobacter intestinal colonisation levels are influenced by the host genetics of the chicken. In the present study, two chicken populations were used to investigate the genetic architecture of avian resistance to colonisation: (i) a back-cross of two White Leghorn derived inbred lines [(6(1) x N) x N] known to differ in resistance to Campylobacter colonisation and (ii) a 9th generation advanced intercross (6(1) x N) line. Results: The level of colonisation with Campylobacter jejuni following experimental infection was found to be a quantitative trait. A back-cross experiment using 1,243 fully informative single nucleotide polymorphism (SNP) markers revealed quantitative trait loci (QTL) on chromosomes 7, 11 and 14. In the advanced intercross line study, the location of the QTL on chromosome 14 was confirmed and refined and two new QTLs were identified located on chromosomes 4 and 16. Pathway and re-sequencing data analysis of the genes located in the QTL candidate regions identified potential pathways, networks and candidate resistance genes. Finally, gene expression analyses were performed for some of the candidate resistance genes to support the results. Conclusion: Campylobacter resistance in chickens is a complex trait, possibly involving the Major Histocompatibility Complex, innate and adaptive immune responses, cadherins and other factors. Two of the QTLs for Campylobacter resistance are co-located with Salmonella resistance loci, indicating that it may be possible to breed simultaneously for enhanced resistance to both zoonoses.

引用

页数：18

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