Leishmania mexicana and Leishmania major:: Attenuation of wild-type parasites and vaccination with the attenuated lines

被引：59

作者：

Daneshvar, H ^{[1
]}

Coombs, GH ^{[1
]}

Hagan, P ^{[1
]}

Phillips, RS ^{[1
]}

机构：

[1] Univ Glasgow, Inst Biomed & Life Sci, Div Infect & Immun, Glasgow G12 8QQ, Lanark, Scotland

来源：

JOURNAL OF INFECTIOUS DISEASES | 2003年 / 187卷 / 10期

关键词：

D O I：

10.1086/374783

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A method for attenuation of Leishmania species by culturing in vitro under gentamicin pressure has been used successfully with Leishmania mexicana, L. major, L. infantum, and L. donovani. The attenuated lines invaded but were unable to survive within bone marrow-derived macrophages in vitro, whereas wild-type parasites survived and multiplied. The attenuated lines of L. mexicana and L. major both failed to induce cutaneous lesions in the majority of BALB/c mice over a minimum 12-week observation period after subcutaneous injection of stationary phase parasites. The attenuated line of L. mexicana retained its properties in gentamicin-free medium over 40 subcultures. The attenuated lines of L. mexicana and L. major both induced significant protection in mice against challenge with wild-type parasites.

引用

页码：1662 / 1668

页数：7

共 30 条

[21]

Rappuoli R, 1998, VACCINES - FROM CONCEPT TO CLINIC, P1

[22] VACCINE DEVELOPMENT AGAINST CUTANEOUS LEISHMANIASIS - SUBCUTANEOUS ADMINISTRATION OF RADIOATTENUATED PARASITES PROTECTS CBA MICE AGAINST VIRULENT LEISHMANIA-MAJOR CHALLENGE [J].

RIVIER, D ;

SHAH, R ;

BOVAY, P ;

MAUEL, J .

PARASITE IMMUNOLOGY, 1993, 15 (02) :75-84

[23] Cultivation of clinically significant hemoflagellates [J].

Schuster, FL ;

Sullivan, JJ .

CLINICAL MICROBIOLOGY REVIEWS, 2002, 15 (03) :374-+

[24] Experimental studies on the evolution of antimony-resistant phenotype during the in vitro life cycle of Leishmania infantum:: implications for the spread of chemoresistance in endemic areas [J].

Sereno, D ;

Guilvard, E ;

Maquaire, S ;

Cavaleyra, M ;

Holzmuller, P ;

Ouaissi, A ;

Lemesre, JL .

ACTA TROPICA, 2001, 80 (03) :195-205

[25] Randomised vaccine trial of single dose of killed Leishmania major plus BCG against anthroponotic cutaneous leishmaniasis in Bam, Iran [J].

Sharifi, I ;

FeKri, AR ;

Aflatonian, MR ;

Khamesipour, A ;

Nadim, A ;

Mousavi, MRA ;

Momeni, AZ ;

Dowlati, Y ;

Godal, T ;

Zicker, F ;

Smith, PG ;

Modabber, F .

LANCET, 1998, 351 (9115) :1540-1543

[26] Drug resistance in Indian visceral leishmaniasis [J].

Sundar, S .

TROPICAL MEDICINE & INTERNATIONAL HEALTH, 2001, 6 (11) :849-854

[27] Selective suppression of interleukin-12 induction after macrophage receptor ligation [J].

Sutterwala, FS ;

Noel, GJ ;

Clynes, R ;

Mosser, DM .

JOURNAL OF EXPERIMENTAL MEDICINE, 1997, 185 (11) :1977-1985

[28] DEVELOPMENT OF A SAFE LIVE LEISHMANIA VACCINE LINE BY GENE REPLACEMENT [J].

TITUS, RG ;

GUEIROSFILHO, FJ ;

DEFREITAS, LAR ;

BEVERLEY, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (22) :10267-10271

[29] Aminoglycoside - RNA interactions [J].

Walter, F ;

Vicens, Q ;

Westhof, E .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1999, 3 (06) :694-704

[30] Antigen presentation by Leishmania mexicana-infected macrophages: Activation of helper T cells by a model parasite antigen secreted into the parasitophorous vacuole or expressed on the amastigote surface [J].

Wolfram, M ;

Fuchs, M ;

Wiese, M ;

Stierhof, YD ;

Overath, P .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1996, 26 (12) :3153-3162

← 1 2 3 →