Evidence for egg-box-compatible interactions in calcium-alginate gels from fiber X-ray diffraction

被引:409
作者
Sikorski, Pawel [1 ]
Mo, Frode
Skjak-Braek, Gudmund
Stokke, Bjlrn T.
机构
[1] Norwegian Univ Sci & Technol, Dept Phys, NO-7491 Trondheim, Norway
[2] Norwegian Univ Sci & Technol, Dept Biotechnol, Norwegian Biopolymer Lab, NO-7491 Trondheim, Norway
关键词
D O I
10.1021/bm0701503
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The structures of guluronic-acid-rich alginate in the acid and calcium forms were investigated using fiber X-ray diffraction. Data recorded for alginate fibers in the acid form show a repeat along the chain axis of c = 0.87 nm, a value that is in agreement with the one measured by Atkins et al. (Biopolymers 1973, 12, 1865) and contradicts a repeat of 0.78 nm recently suggested by Li et al. (Biomacromolecules 2007, 8, 464). In the Ca2+ form, our observations indicate that the junction zone involves dimerization of polymer chains through Ca2+ coordination according to the egg-box model. For reasons that are not understood at present, coordination of the divalent cations reduces the ability for the lateral crystallographic packing of the dimers. A proposed model for the junction zone involves polymer chains packed on a hexagonal lattice with a lattice constant a = 0.66 nm. Random pairs of chains form dimers through coordination of Ca2+ cations. Further lateral interaction between dimers is mediated by disordered Na+ and Ca2+ cations, water molecules, and hydrogen bonding.
引用
收藏
页码:2098 / 2103
页数:6
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