Sphingosine inhibits the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) activity

被引：7

作者：

Benaim, Gustavo ^{[1
,2
]}

Pimentel, Adriana A. ^{[3
]}

Felibertt, Pimali ^{[4
]}

Mayora, Adriana ^{[2
]}

Colman, Laura ^{[5
]}

Sojo, Felipe ^{[2
]}

Rojas, Hector ^{[6
,7
]}

De Sanctis, Juan B. ^{[6
]}

机构：

[1] Inst Estudios Avanzados IDEA, Carretera Nacl Hoyo de la Puerta, Caracas, Venezuela

[2] Cent Univ Venezuela, Fac Ciencias, Inst Expt Biol, Caracas, Venezuela

[3] Cent Univ Venezuela, Fac Farm, Caracas, Venezuela

[4] Univ Carabobo, Fac Ciencias, Valencia, Venezuela

[5] Inst Pasteur Montevideo, Montevideo, Uruguay

[6] Cent Univ Venezuela, Inst Inmunol, Caracas, Venezuela

[7] Inst Venezolano Invest Cient, Ctr Biofis & Bioquim, Caracas, Venezuela

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 473卷 / 02期

关键词：

Sphingolipid; Sphingosine; Ca+2; Sarco(endo)plasmic reticulum Ca+2-ATPase; SERCA; MEMBRANE CALCIUM-PUMP; JURKAT T-CELLS; INTRACELLULAR STORES; STRUCTURAL BASIS; CA2+; ACTIVATION; APOPTOSIS; CERAMIDE; PHOSPHOLAMBAN; HOMEOSTASIS;

D O I：

10.1016/j.bbrc.2016.03.123

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The increase in the intracellular Ca2+ concentration ([Ca2+](i)) is the key variable for many different processes, ranging from regulation of cell proliferation to apoptosis. In this work we demonstrated that the sphingolipid sphingosine (Sph) increases the [Ca2+](i) by inhibiting the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA), in a similar manner to thapsigargin (Tg), a specific inhibitor of this Ca2+ pump. The results showed that addition of sphingosine produced a release of Ca2+ from the endoplasmic reticulum followed by a Ca2+ entrance from the outside mileu. The results presented in this work support that this sphingolipid could control the activity of the SERCA, and hence sphingosine may participate in the regulation of [Ca2+](i) in mammalian cells. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：572 / 577

页数：6

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