miR-4417 targets lncRNA PSMG3-AS1 to suppress cell invasion and migration in cervical squamous cell carcinoma

被引：5
作者：
Man, Shuhong [1 ]
Li, Xiaohan [2 ]
Zhu, Wei [3 ]
机构：
[1] Weifang Peoples Hosp, Dept Gynaecol, Weifang 261000, Shandong, Peoples R China
[2] Shandong Univ, Sch Publ Hlth, Jinan 250012, Shandong, Peoples R China
[3] Weifang Peoples Hosp, Nursing Dept, 151 Guangwen St, Weifang 261000, Shandong, Peoples R China
来源：
ONCOLOGY LETTERS | 2021年 / 22卷 / 01期
关键词：
CSCC; lncRNA PSMG3-AS1; microRNA-4417; invasion; migration; CANCER; WOMEN;
D O I：
10.3892/ol.2021.12763
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Although long non-coding RNA (lncRNA) PSMG3-AS1 has been reported to participate in cancer biology, its role in cervical squamous cell carcinoma (CSCC) is unknown. The present study aimed to investigate the role of the lncRNA PSMG3-AS1 in CSCC. The expression levels of PSMG3-AS1 in both CSCC and non-tumor tissues from 64 patients with CSCC were measured by reverse transcription-quantitative PCR. The potential interaction between miR-4417 and PSMG3-AS1 was predicted using IntaRNA 2.0. Overexpression of miR-4417 and PSMG3-AS1 were achieved in CSCC cells to further explore the potential interaction between them. The effects of overexpression of miR-4417 and PSMG3-AS1 on CSCC cell invasion and migration were assessed by Transwell assay. The results revealed that PSMG3-AS1 expression was upregulated in CSCC tissues, and its high expression levels predicted a poor survival in patients with CSCC. miR-4417 expression was downregulated in CSCC tissues and was inversely correlated with PSMG3-AS1 expression. Moreover, miR-4417 was predicted to interact with PSMG3-AS1. In CSCC cells, overexpression of miR-4417 decreased the expression levels of PSMG3-AS1, while overexpression of PSMG3-AS1 did not affect miR-4417 expression. Transwell assay demonstrated that overexpression of PSMG3-AS1 increased CSCC cell invasion and migration. However, overexpression of miR-4417 inhibited CSCC cell invasion and migration, and attenuated the effects of PSMG3-AS1 overexpression in CSCC cells. In conclusion, the present study indicated that miR-4417 may target PSMG3-AS1 to suppress cancer cell invasion and migration in CSCC.
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页数：7
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共 17 条
[1]   The HPV-18 E7 CKII phospho acceptor site is required for maintaining the transformed phenotype of cervical tumour-derived cells [J].
Basukala, Om ;
Mittal, Suruchi ;
Massimi, Paola ;
Bestagno, Marco ;
Banks, Lawrence .
PLOS PATHOGENS, 2019, 15 (05)
[2]  
Bray F, 2018, CA-CANCER J CLIN, V68, P394, DOI [10.3322/caac.21492, 10.3322/caac.21609]
[3]   Beyond Chemotherapy: An Overview and Review of Targeted Therapy in Cervical Cancer [J].
Crafton, Sarah M. ;
Salani, Ritu .
CLINICAL THERAPEUTICS, 2016, 38 (03) :449-458
[4]   Novel lncRNA PSMG3-AS1 functions as a miR-143-3p sponge to increase the proliferation and migration of breast cancer cells [J].
Cui, Yue ;
Fan, Yuhua ;
Zhao, Guangcai ;
Zhang, Qibing ;
Bao, Ying ;
Cui, Yuanri ;
Ye, Zengjie ;
Chen, Guoyou ;
Piao, Xianji ;
Guo, Fang ;
Wang, Jinghao ;
Bai, Yuhua ;
Yu, Dejun .
ONCOLOGY REPORTS, 2020, 43 (01) :229-239
[5]   E6 proteins from high-risk HPV, low-risk HPV, and animal papillomaviruses activate the Wnt/-catenin pathway through E6AP-dependent degradation of NHERF1 [J].
Drews, Camille M. ;
Case, Samuel ;
Vande Pol, Scott B. .
PLOS PATHOGENS, 2019, 15 (04)
[6]   Potential of long non-coding RNAs in cancer patients: From biomarkers to therapeutic targets [J].
Gupta, Subash Chandra ;
Tripathi, Yashoda Nandan .
INTERNATIONAL JOURNAL OF CANCER, 2017, 140 (09) :1955-1967
[7]   Molecular pathogenesis of cervical cancer [J].
Ibeanu, Okechukwu A. .
CANCER BIOLOGY & THERAPY, 2011, 11 (03) :295-306
[8]   Cervical Cancer, Version 2.2015 Featured Updates to the NCCN Guidelines [J].
Koh, Wui-Jin ;
Greer, Benjamin E. ;
Abu-Rustum, Nadeem R. ;
Apte, Sachin M. ;
Campos, Susana M. ;
Cho, Kathleen R. ;
Chu, Christina ;
Cohn, David ;
Crispens, Marta Ann ;
Dorigo, Oliver ;
Eifel, Patricia J. ;
Fisher, Christine M. ;
Frederick, Peter ;
Gaffney, David K. ;
Han, Ernest ;
Huh, Warner K. ;
Lurain, John R., III ;
Mutch, David ;
Fader, Amanda Nickles ;
Remmenga, Steven W. ;
Reynolds, R. Kevin ;
Teng, Nelson ;
Tillmanns, Todd ;
Valea, Fidel A. ;
Yashar, Catheryn M. ;
McMillian, Nicole R. ;
Scavone, Jillian L. .
JOURNAL OF THE NATIONAL COMPREHENSIVE CANCER NETWORK, 2015, 13 (04) :395-404
[9]   AJCC versus FIGO staging of cervical cancer to predict disease severity [J].
Liu, C. S. ;
Spirtos, A. ;
Khulpateea, B. R. ;
Lea, J. S. .
GYNECOLOGIC ONCOLOGY, 2018, 149 :155-155
[10]   Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].
Livak, KJ ;
Schmittgen, TD .
METHODS, 2001, 25 (04) :402-408
12