Biliverdin administration ameliorates cerebral ischemia reperfusion injury in rats and is associated with proinflammatory factor downregulation

被引:31
作者
Li, Jun-Jie [1 ]
Zou, Zhi-Yao [1 ]
Liu, Jia [2 ]
Xiong, Liu-Lin [3 ,4 ]
Jiang, Hai-Yan [1 ]
Wang, Ting-Hua [2 ,3 ,4 ]
Shao, Jian-Lin [1 ]
机构
[1] Kunming Med Univ, Affiliated Hosp 1, Dept Anesthesiol, 295 Xichang Rd, Kunming 650032, Yunnan, Peoples R China
[2] Kunming Med Univ, Inst Neurosci, Anim Ctr, Kunming 650031, Yunnan, Peoples R China
[3] Sichuan Univ, West China Hosp, Dept Anesthesiol, Translat Neurosci Ctr, 17 Sect 3,South Renmin Rd, Chengdu 610041, Sichuan, Peoples R China
[4] Sichuan Univ, West China Hosp, Inst Neurol Dis, Translat Neurosci Ctr, 17 Sect 3,South Renmin Rd, Chengdu 610041, Sichuan, Peoples R China
关键词
biliverdin; cerebral ischemia; reperfusion injury; anti-inflammation; HEME OXYGENASE-1; INFLAMMATORY MEDIATORS; ISCHEMIA/REPERFUSION INJURY; ARTERY OCCLUSION; BRAIN-DAMAGE; OXIDATIVE DAMAGE; FOCAL ISCHEMIA; UP-REGULATION; EXPRESSION; PROTECTS;
D O I
10.3892/etm.2017.4549
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Biliverdin (BV), one of the heme oxygenase-1 (HO-1) catalytic products, has been demonstrated to have protective effects in liver ischemia reperfusion injury (IRI). The present study aimed to explore the effects of BV on cerebral IRI, and to investigate the potential mechanisms thereof. Adult male SD rats, weighing 200-240 g, were randomly divided into sham (group S), cerebral ischemia reperfusion control (group C) and BV (group BV) groups. Rats in group C underwent transient middle cerebral artery occlusion (tMCAO) and received 2 ml normal saline; rats in group BV received BV (35 mg/kg) intraperitoneally 15 min prior to reperfusion and 4 h after reperfusion, then twice a day thereafter for 5 days. Group S served as the control. Neurological Severity Scores (NSS) were evaluated at days 1-5 following reperfusion. Staining with 2, 3, 5-triphenyltetrazolium chloride was performed to determine the cerebral infarction at 48 h post reperfusion. mRNA expression levels of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, IL-1 beta, inducible nitric oxide synthase (iNOS) and HO-1 in the ischemic cerebral cortex were detected via reverse transcription-quantitative polymerase chain reaction at 3, 6, 12 and 24 h after reperfusion. Western blotting was used to detect the protein expression levels at 3 h after reperfusion. Compared with group S, the NSS, cerebral infarct volume, and the mRNA and protein expression levels of TNF-alpha, IL-6, IL-1 beta, iNOS and HO-1 of Group C were significantly increased (P<0.05). However, BV administration significantly improved and reduced these expression levels (P<0.01). The present study indicates that BV is able to ameliorate cerebral IRI in rats and that the mechanism may be associated with the downregulation of proinflammatory factors.
引用
收藏
页码:671 / 679
页数:9
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