Higher dimensional (Hi-D) separation strategies dramatically improve the potential for cancer biomarker detection in serum and plasma

被引：65
作者：
Hoffman, Seth A. [1 ]
Joo, Won-A. [1 ]
Echan, Lynn A. [1 ]
Speicher, David W. [1 ]
机构：
[1] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 849卷 / 1-2期
关键词：
cancer; proteomics; plasma proteome; serum proteome; blood proteome; multidimensional separation; biomarkers; protein separation; peptide separation;
D O I：
10.1016/j.jchromb.2006.10.069
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The plasma proteome has a wide dynamic range of protein concentrations and is dominated by a few highly abundant proteins. Discovery of novel cancer biomarkers using proteomics is particularly challenging because specific biomarkers are expected to be low abundance proteins with normal blood concentrations of low nanograms per milliliter or less. Conventional, one- and two-dimensional proteomic methods including 2D PAGE, 2D DIGE, LC-MS/MS, and LC/LC-MS/MS do not have the capacity to consistently detect many proteins in this range. In contrast, new higher dimensional (Hi-D) separation strategies, utilizing more than two dimensions of fractionation, can profile the low abundance proteome. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：43 / 52
页数：10
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