Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations

被引：696
作者：
Sekar, RB
Periasamy, A
机构：
[1] Univ Virginia, Dept Biol, WM Keck Ctr Cellular Imaging, Charlottesville, VA 22904 USA
[2] Univ Virginia, Dept Biomed Engn, WM Keck Ctr Cellular Imaging, Charlottesville, VA 22904 USA
来源：
JOURNAL OF CELL BIOLOGY | 2003年 / 160卷 / 05期
关键词：
FRET microscopy; data analysis; cameleons; dimerization; FRET assays;
D O I：
10.1083/jcb.200210140
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.
引用
收藏
页码：629 / 633
页数：5
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