Isolation and characterization of ent-pimarane diterpenoids from Sigesbeckia pubescens

被引：282
作者：
Wang, Jianbin [1 ,2 ,3 ]
Xie, Kehui [3 ]
Wang, Qing [3 ]
Li, Wenchao [3 ]
Fu, Hongzheng [3 ]
机构：
[1] Jinan Univ, Coll Pharm, Res Ctr Tradit Chinese Med Lingnan Southern China, Guangzhou, Guangdong, Peoples R China
[2] Guangdong Macau Tradit Chinese Med Technol Ind Pk, Zhuhai, Peoples R China
[3] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing, Peoples R China
来源：
NATURAL PRODUCT RESEARCH | 2021年 / 35卷 / 09期
基金：
美国国家科学基金会; 中国博士后科学基金;
关键词：
ent-Pimarane diterpenoids; Sigesbeckia; Sigesbeckia pubescens Makino; Snatzke's method; anti-inflammatory; RAW; 264; 7 macrophage cells; COLLAGEN-INDUCED ARTHRITIS; NF-KAPPA-B; NITRIC-OXIDE; SIEGESBECKIA; INHIBITION; ACTIVATION; KAURANE;
D O I：
10.1080/14786419.2019.1656627
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Five new ent-pimarane diterpenoids ent-16-nor-2-oxopimar-8(14)-ene-15,19-dial (1), ent-16-nor-2 alpha,19-dihydroxypimar-8-en-15-al (2), 3-O-acetyldarutigenol (3), 19-O-acetylkirenol (4), ent-16-nor-3 beta,15-dihydroxypimar-8(14)-ene (5) were isolated and characterized from the ethanol extract of Sigesbeckia pubescens. Their structures were elucidated on the basis of spectroscopic analysis. The absolute configuration of C-15 in compounds 3 and 4 was assigned using Snatzke's method. All these compounds were assessed for their anti-inflammatory potential by measuring the inhibitory effects on NO production in LPS-induced RAW 264.7 macrophage cells and compound 4 showed significantly inhibitory activity with IC50 value of 5.9 mu M.
引用
收藏
页码：1510 / 1517
页数：8
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