Interactions of Calmodulin With the Multiple Binding Sites of Cav1.2 Ca2+ Channels

被引：37

作者：

Asmara, Hadhimulya ^{[1
]}

Minobe, Etsuko ^{[1
]}

Saud, Zahangir A. ^{[1
]}

Kameyama, Masaki ^{[1
]}

机构：

[1] Kagoshima Univ, Dept Physiol, Grad Sch Med & Dent Sci, Kagoshima 8908544, Japan

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2010年 / 112卷 / 04期

基金：

日本学术振兴会;

关键词：

calcium channel; calmodulin; ion channel regulation; IQ motif; cardiac myocyte; CALCIUM-CHANNELS; CA2+-SENSITIVE INACTIVATION; CA2+-DEPENDENT INACTIVATION; ALPHA(1C) SUBUNIT; CRYSTAL-STRUCTURE; CA(V)1.2 CHANNEL; FACILITATION; DOMAIN; MOTIF; DETERMINANTS;

D O I：

10.1254/jphs.09342FP

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Although calmodulin binding to various sites of the Cav1.2 Ca2+ channel has been reported, the mechanism of the interaction is not fully understood. In this study we examined calmodulin binding to fragment channel peptides using a semi-quantitative pull-down assay. Calmodulin bound to the peptides with decreasing affinity order: IQ > preIQ > I-II loop > N-terminal peptide. A peptide containing both preIQ and IQ regions (Leu(1599) - Leu(1668)) bound with approximately 2 mol of calmodulin per peptide. These results support the hypothesis that two molecules of calmodulin can simultaneously bind to the C-terminus of the Cav1.2 channel and modulate its facilitatory and inhibitory activities.

引用

页码：397 / 404

页数：8

共 29 条

[1] Calmodulin interactions with IQ peptides from voltage-dependent calcium channels [J].

Black, DJ ;

Halling, DB ;

Mandich, DV ;

Pedersen, SE ;

Altschuld, RA ;

Hamilton, SL .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2005, 288 (03) :C669-C676

[2] ESSENTIAL CA2+-BINDING MOTIF FOR CA2+-SENSITIVE INACTIVATION OF L-TYPE CA2+ CHANNELS [J].

DELEON, M ;

WANG, Y ;

JONES, L ;

PEREZREYES, E ;

WEI, XY ;

SOONG, TW ;

SNUTCH, TP ;

YUE, DT .

SCIENCE, 1995, 270 (5241) :1502-1506

[3] A modular switch for spatial Ca2+ selectivity in the calmodulin regulation of CaV channels [J].

Dick, Ivy E. ;

Tadross, Michael R. ;

Liang, Haoya ;

Tay, Lai Hock ;

Yang, Wanjun ;

Yue, David T. .

NATURE, 2008, 451 (7180) :830-U9

[4] Cloning and expression of the Ca2+ channel α1C and β2a subunits from guinea pig heart [J].

Ding, S ;

Kuroki, S ;

Kameyama, A ;

Yoshimura, A ;

Kameyama, M .

JOURNAL OF BIOCHEMISTRY, 1999, 125 (04) :750-759

[5] RETRACTED: Calmodulin kinase determines calcium-dependent facilitation of L-type calcium channels (Retracted Article) [J].

Dzhura, I ;

Wu, YJ ;

Colbran, RJ ;

Balser, JR ;

Anderson, ME .

NATURE CELL BIOLOGY, 2000, 2 (03) :173-177

[6] FRET two-hybrid mapping reveals function and location of L-type Ca2+ channel CaM preassociation [J].

Erickson, MG ;

Liang, HY ;

Mori, MX ;

Yue, DT .

NEURON, 2003, 39 (01) :97-107

[7] Crystal structure of dimeric cardiac L-type calcium channel regulatory domains bridged by Ca2+•calmodulins [J].

Fallon, Jennifer L. ;

Baker, Mariah R. ;

Xiong, Liangwen ;

Loy, Ryan E. ;

Yang, Guojun ;

Dirksen, Robert T. ;

Hamilton, Susan L. ;

Quiocho, Florante A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (13) :5135-5140

[8] Structure of calmodulin bound to the hydrophobic IQ domain of the cardiac Cav1.2 calcium channel [J].

Fallon, JL ;

Halling, DB ;

Hamilton, SL ;

Quiocho, FA .

STRUCTURE, 2005, 13 (12) :1881-1886

[9]

Halling D.B., 2005, Sci STKE, V315, P1

[10] Calmodulin- and Ca2+-Dependent Facilitation and Inactivation of the CaV1.2 Ca2+ Channels in Guinea-Pig Ventricular Myocytes [J].

Han, Dong-Yun ;

Minobe, Etsuko ;

Wang, Wu-Yang ;

Guo, Feng ;

Xu, Jian-Jun ;

Hao, Li-Ying ;

Kameyama, Masaki .

JOURNAL OF PHARMACOLOGICAL SCIENCES, 2010, 112 (03) :310-319

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