High-resolution melting analysis of ADAMTS18 methylation levels in gastric, colorectal and pancreatic cancers

被引：41

作者：

Li, Zhi ^{[2
,3
]}

Zhang, Wei ^{[4
]}

Shao, Yong ^{[1
,5
]}

Zhang, Chao ^{[1
]}

Wu, Qi ^{[1
]}

Yang, Hong ^{[5
]}

Wan, Xiangbin ^{[3
]}

Zhang, Jie ^{[5
]}

Guan, Ming ^{[6
]}

Wan, Jun ^{[1
,7
]}

Yu, Bo ^{[1
,5
]}

机构：

[1] Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen, Guangdong, Peoples R China

[2] Zhengzhou Univ, Basic Med Coll, Dept Histol & Embryol, Zhengzhou, Henan, Peoples R China

[3] Henan Prov Tumor Hosp, Dept Gen Surg, Zhengzhou, Henan, Peoples R China

[4] Ji Nan Univ, HKUST JNU Joint Lab, Guangzhou, Guangdong, Peoples R China

[5] Peking Univ, Shenzhen Hosp, Dept Dermatol, Shen Zhen, Guangdong, Peoples R China

[6] Fudan Univ, Huashan Hosp, Cent Lab, Dept Lab Med, Shanghai 200433, Peoples R China

[7] Hong Kong Univ Sci & Technol, Dept Biochem, Kowloon, Hong Kong, Peoples R China

来源：

MEDICAL ONCOLOGY | 2010年 / 27卷 / 03期

关键词：

ADAMTS18; Methylation; HRM; Gastric cancer; Colorectal cancer; Pancreatic cancer; DNA METHYLATION; EMERGING ROLES; CELL-LINES; PCR BIAS; FAMILY; GENE; METALLOPROTEINASES; NASOPHARYNGEAL; IDENTIFICATION; AGGRECANASE-1;

D O I：

10.1007/s12032-009-9323-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A disintegrin and metalloprotease with trombo-spondin motifs (ADAMTS) is a family of proteins characterized by the presence of a metalloproteinase domain linked to a variety of specialized ancillary domains. ADAMTS18 is a putative tumor suppressive gene related to nasopharyngeal carcinoma. We used high-resolution melting (HRM) analysis to detect the methylation levels of ADAMTS18 gene in 100 gastric cancers, 100 colorectal cancers, 70 pancreatic cancers, and equal number of adjacent normal tissues. The frequency of ADAMTS18 methylation in all three types of cancers was significantly higher than that in normal tissues. Expression levels of ADAMTS18 were inversely correlated with methylation levels. No significant association was found between ADAMTS18 methylation status and TNM staging in the cancers. In summary, epigenetic regulation of ADAMTS18 was associated with carcinogenesis. The application of HRM analysis is a fast and high-throughput way to investigate the epigenetic status of ADAMTS18.

引用

页码：998 / 1004

页数：7

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