Ratiometric Two-Photon Fluorescent Probe for Detecting and Imaging Hypochlorite

被引：95

作者：

Pak, Yen Leng ^{[1
]}

Park, Sang Jun ^{[2
]}

Xu, Qingling ^{[3
]}

Kim, Hwan Myung ^{[2
]}

Yoon, Juyoung ^{[1
]}

机构：

[1] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea

[2] Ajou Univ, Dept Energy Syst Res, Suwon 443749, Gyeonggi Do, South Korea

[3] Univ Chinese Acad Sci, Sch Chem Sci, Beijing 100049, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2018年 / 90卷 / 15期

基金：

新加坡国家研究基金会; 中国国家自然科学基金;

关键词：

SINGLE-WAVELENGTH EXCITATION; LIVING CELLS; LIVE CELLS; IN-VIVO; CHLORINATING ACTIVITY; ACID; MYELOPEROXIDASE; PH; HOCL; MITOCHONDRIA;

D O I：

10.1021/acs.analchem.8b02195

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The ratiometric fluorescent probe B6S, which contains pyrene as a fluorophore and imidazoline-2-thione as a reactive site, was developed for detection of hypochlorite (OCl-). B6S displays a high specificity toward OCl(- )in contrast to other reactive oxygen species and reactive nitrogen species. The probe has a low detection limit and operates under biological conditions. Moreover, the low cytotoxicity of B6S enables it to be utilized effectively for OCl- imaging in living cells and tissues by using two-photon microscopy. The findings indicate that B6S has the capability of serving as a probe to explore the biological functions of OCl- in living systems.

引用

页码：9510 / 9514

页数：5

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